Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin

Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media. The matrix is a 6% crosslinked agarose with optimal properties for batch, column-based, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of Pierce high-Capacity EDTA-Compatible Ni-IMAC Resin

• Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
• Versatile—purify both overexpressed and secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
• Reusable—loose resin and spin column formats can be regenerated up to 10 times using the standard CIP protocol (not applicable to 96-well plates)
• Flexible— available in multiple formats including bulk loose resin, spin columns, and 96-well spin plates

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping off the metal. Because of its chelator stability characteristic (EDTA tolerant), Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is specially suited for purification of expressed His-tagged proteins that are secreted into cell culture media or intracellular His-tagged proteins that need the presence of EDTA to maintain stability and function. In both cases, large quantities of samples can be purified without the Ni metal stripping off of the support, thereby providing consistent high-capacity yields.

Available formats

• Resin slurries—20 mL, 100 mL, 500 mL, and 1 L of 50% slurry in 20% ethanol
• Spin columns—10 mL spin columns containing 1 mL of settled resin, supplied as 5 mL of 20% slurry
• 96-well spin plates—96-well spin plates with 50 μL of settled resin, supplied as 250 μL of 20% slurry