Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS

Catalog number: A43841
Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS

Catalog number: A43841
Catalog Number
A43841
Unit Size
200 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A43841200 mL
-
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This substrate kit is comprised of SuperSignal West Femto Maximum Sensitivity Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both high sensitivity and routine western blotting.

SuperSignal West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult to detect targets.

Sensitivity: low-femtogram (mid-zeptomole)
Stability: 8-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: up to 8 hours
Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

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SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets.

Sensitivity: pictogram to femtogram sensitivity
Stability: 8-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 6 to 24 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Substrate
Detection Method
Chemiluminescent
Form
Liquid
For Use With (Application)
Western Blot
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Quantity
1 Bundle
Substrate
Horseradish Peroxidase
Sensitivity
Picogram to femtogram, low femtogram (mid-zeptomole)
Sufficient For
60 Mini-Gel Blots, 5000 sq. cm of membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Shipping Condition
Room Temperature

Contents & Storage

• 200 mL SuperSgnal West Femto reagent, store at 2–8°C
• 20 mL SuperSignal West Pico PLUS reagent, store at room temperature

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