SuperSignal™ West Pico PLUS Chemiluminescent Substrate
SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogramRead more
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Catalog NumberQuantity
34577200 mL
3457920 mL
34580500 mL
34580X44 x 500 mL
345781 L
34578X44 x 1 L
Catalog number 34577
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187.65
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204.00
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Quantity:
200 mL
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Price (USD)/ Each
187.65
Online Exclusive
204.00
Save 16.35 (8%)
Add to cart

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

  • Sensitivity: pictogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates

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SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity200 mL
ConjugateHRP
SubstrateHorseradish Peroxidase
Recommended Antibody Concentrations2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
SensitivityPicogram to high femtogram-level
Signal Duration6 to 24 hr.
Sufficient For25 Mini-Gel Blots, 2000 cm2 of Membrane
Kit ContentsSuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Sufficient for 25 mini-gel blots; 2000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
• SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Store at room temperature.