Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number: 34577
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number: 34577
Catalog Number
34577
Unit Size
200 mL
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Availability
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Unit Size
20 mL
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1 L
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500 mL
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4 x 500 mL
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Unit Size
4 x 1 L
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34577200 mL
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3457920 mL
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345781 L
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34580500 mL
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34580X44 x 500 mL
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34578X44 x 1 L
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Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
Sensitivity: pictogram to femtogram sensitivity
Stability: 8-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 6 to 24 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
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SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Substrate
Detection Method
Chemiluminescent
Form
Liquid
For Use With (Application)
Western Blot
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Quantity
200 mL
Conjugate
HRP
Substrate
Horseradish Peroxidase
Recommended Antibody Concentrations
2°: 1:20-100K (10-50ng/mL), 1°: 1:1K (0.2- 1μg/mL)
Sensitivity
Picogram to high femtogram-level
Signal Duration
6 to 24 hr.
Sufficient For
25 Mini-Gel Blots, 2000 sq. cm of Membrane
Kit Contents
SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL, SuperSignal West Pico PLUS Stable Peroxide, 100 mL
Shipping Condition
Room Temperature

Contents & Storage

Sufficient for 25 mini-gel blots; 2000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
• SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Store at room temperature.

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