Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog number: 34094
Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog number: 34094
Catalog Number
34094
Unit Size
20 mL
Price (USD)
Price: 128.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
100 mL
Price (USD)
Price: 398.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
200 mL
Price (USD)
Price: 652.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
3409420 mL
Price: 128.00
Your Price:
-
34095100 mL
Price: 398.00
Your Price:
-
34096200 mL
Price: 652.00
Your Price:
-
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Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
Sensitivity: low-femtogram (mid-zeptomole)
Stability: 8-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: up to 8 hours
Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
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SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Chemiluminescent
Form
Liquid
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Product Type
Substrate
Quantity
20 mL
Sensitivity
low femtogram-level
Substrate
Horseradish Peroxidase
Substrate Properties
Chemical Substrate
Substrate Type
HRP Substrate
Sufficient For
2 Mini-Gel Blots, 200 sq. cm of Membrane
Validated Application
Western Blotting
Kit Contents
Stable Peroxide Buffer, 10mL, Luminol/Enhancer, 10mL
Recommended Antibody Concentrations
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration
6 to 8 hr

Contents & Storage

Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
• Luminol/Enhancer, 10 mL
• Stable Peroxide Buffer, 10 mL

Store reagents at 4°C.