Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog number:  34094

Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
Sensitivity: low-femtogram (mid-zeptomole)
Stability: 8-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: up to 8 hours
Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

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SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration: 6 to 8 hr
Detection Method: Chemiluminescent
Form: Liquid
Product Line: SuperSignal™
Quantity: 20 mL Kit
Substrate: Horseradish Peroxidase
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Kit Contents: Stable Peroxide Buffer, 10mL
Product Type: Substrate
Sensitivity: Low to mid-femtogram

Contents & storage

Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
• Luminol/Enhancer, 10 mL
• Stable Peroxide Buffer, 10 mL

Store reagents at 4°C.