Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Catalog number: A45915
Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Catalog number: A45915
Catalog Number
A45915
Unit Size
500 mL + 20 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A45915500 mL + 20 mL
-
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This substrate bundle contains SuperSignal West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for routine western blotting applications.

• Sensitivity: picogram to femtogram
• Stability: eight hours for working solution; one year for kit at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Substrate
Detection Method
Chemiluminescent
Form
Liquid
For Use With (Application)
Western Blot
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Quantity
1 Bundle
Substrate
Horseradish Peroxidase
Sensitivity
Picogram to femtogram, low-femtogram to high-attogram
Sufficient For
12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Shipping Condition
Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & Storage

• 500 mL SuperSignal West Pico PLUS reagent, store at room temperature
• 20 mL SuperSignal West Atto reagent, store at 2–8°C

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