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FIGURE: 1 / 4
Human IgG (Lambda Light Chain) Secondary Antibody (A56867) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized HEK293E cells that were transfected to overexpress SARS-CoV-2 Spike Protein (RBD) Recombinant Human Monoclonal Antibody (P06DHu) (Product # 703973, Lambda Light chain containing mAb) and detected using Goat-anti-Human IgG (Lambda Light Chain), Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A56867, 1:1,000). Panel a) shows... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A56867
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:1,000 - 1:2,000
Flow Cytometry (Flow)
1:1,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
85H45L7
Immunogen
Purified Human IgG
Conjugate
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
250 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.5
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Target
IgG (Lambda light chain)
Antibody Form
Whole Antibody
Product Specific Information
This Goat-anti-Human IgG (Lambda Light Chain), Recombinant Secondary Antibody specifically detects human IgG Lambda Light chain with no cross-reactivity to kappa light chain, heavy chain, and other species immunoglobulins.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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