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Bethyl Laboratories
SMAD2 Recombinant Rabbit Monoclonal Antibody (BLR130H)
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Product Details
A700-130
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR130H
Immunogen
Peptide representing a region between residue 225 and 275 of Human Mothers against decapentaplegic homolog 2 (aka SMAD2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
500 µg/mL
Amount
50 µg
Purification
purified
Storage buffer
BBS, pH 8.2, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAD; MAD homolog 2; Mad protein homolog; mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD; Smad 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3; unnamed protein product
Gene Aliases: 7120426M23Rik; CHTD8; hMAD-2; hSMAD2; JV18; JV18-1; LDS6; MADH2; MADR2; mMad2; Smad-2
UniProt ID: (Human) Q15796, (Mouse) Q62432
Entrez Gene ID: (Human) 4087, (Mouse) 17126
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
phosphatase binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
SMAD binding
tau protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
disordered domain specific binding
DNA-binding transcription factor binding
ureteric bud development
mesoderm formation
regionalization
determination of left/right asymmetry in lateral mesoderm
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
gastrulation
mesoderm development
anatomical structure morphogenesis
embryonic pattern specification
anterior/posterior pattern specification
negative regulation of gene expression
positive regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
cell differentiation
negative regulation of ossification
positive regulation of BMP signaling pathway
primary miRNA processing
activin receptor signaling pathway
intracellular signal transduction
nodal signaling pathway
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
regulation of multicellular organismal process
SMAD protein signal transduction
mesenchyme development
trophoblast cell migration
secondary palate development
response to cholesterol
odontoblast differentiation
skeletal system development
in utero embryonic development
endoderm formation
protein phosphorylation
pattern specification process
endoderm development
heart development
cell proliferation
negative regulation of cell proliferation
response to glucose
post-embryonic development
positive regulation of gene expression
signal transduction involved in regulation of gene expression
insulin secretion
lung development
adrenal gland development
pancreas development
organ growth
developmental growth
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
pericardium development
response to transforming growth factor beta
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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