CellBlox™ Blocking Buffer
This product will be discontinued on July 15, 2024. Please use CellBlox Plus Blocking Buffer (Catalog numbers C001T02F01, C001T03F01, C001T06F01) as an enhanced replacement product.
CellBlox™ Blocking Buffer
Invitrogen™

CellBlox™ Blocking Buffer

The Invitrogen™ CellBlox™ Blocking Buffer is formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells. These nonspecific interactionsRead more
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Catalog NumberQuantity
B001T06F01500 Tests
B001T02F0125 Tests
B001T03F01100 Tests
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Catalog number B001T06F01
Price (USD)/ 500 tests
473.00
-
Add to cart
Quantity:
500 Tests
Price (USD)/ 500 tests
473.00
Add to cart
CellBlox™ Blocking Buffer
Catalog numberB001T06F01
Price (USD)/ 500 tests
473.00
-
Add to cart

The Invitrogen™ CellBlox™ Blocking Buffer is formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells. These nonspecific interactions can result in higher background labeling. CellBlox Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling. (Figure 1)

CellBlox Blocking Buffer is also recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling. (Figure 2)

Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols. Add 5 μL CellBlox Blocking Buffer directly to a cell suspension containing 103 - 108 cells prior to the addition of an antibody, with 100 μL as a final staining volume. CellBlox Blocking Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 μL CellBlox Blocking Buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 μL as a final staining volume.

• Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction.
• CellBlox Blocking Buffer is compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains.
• CellBlox Blocking Buffer can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution.
• CellBlox Blocking Buffer is compatible with other blocking reagents, such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer.
• CellBlox Blocking Buffer is not required when labeling antibody-capture beads

Specifications
DescriptionBlocking Buffer
For Use With (Application)Flow Cytometry
Quantity500 Tests
Unit Size500 tests