Bolt™ Transfer Buffer (20X)

Catalog number:  BT0006

 Related applications:

Protein Assays and Analysis

| Protein Gel Electrophoresis

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Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Mini Blot Module for wet transfer.

Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes.

About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Buffer Type: Transfer Buffers
Concentrated: 20 X
Gel Compatibility: Bolt™ Bis-Tris Plus Gels
Product Line: Bolt®
Product Size: 125 mL
Volume (Metric): 125 ml

Contents & storage

Store at 4 to 25°C.


Manuals & protocols