Sondas fluorescentes CellTracker™

Citas y referencias (13)

Invitrogen™

Sondas fluorescentes CellTracker™

Name: Sondas fluorescentes CellTracker™
SKU: C12881

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.

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Número de catálogo	Cantidad	Tipo de colorante
C12881	5 mg	CellTracker Blue CMF2HC
C2110	5 mg	CellTracker™ Blue CMAC
C2111	5 mg	CellTracker™ Blue CMHC
C34565	20 × 15 μg	CellTracker™ Deep Red
C7025	20 x 50 μg	CellTracker Green CMFDA
C2925	1mg	CellTracker Green CMFDA
C2102	5 mg	Colorantes BODIPY
C34551	20 × 50 μg	CellTracker Orange CMRA
C2927	1 mg	CellTracker Orange CMTMR
C34552	20 × 50 μg	CellTracker™ Red CMTPX
C10094	5 x 0,1 mg	ThiolTracker™ Violet

11 opciones

Número de catálogo C12881

Precio (MXN)

Cantidad:

5 mg

Tipo de colorante:

CellTracker Blue CMF2HC

Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorAzul

DescripciónCellTracker™ Blue CMF2HC Dye, 5 mg

Tipo de coloranteCellTracker Blue CMF2HC

Emisión464 nm

Intervalo de longitud de onda de excitación371⁄464

FormularioDry Powder

Línea de productosCellTracker

Cantidad5 mg

Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaOtras etiquetas o colorantes

Tipo de productoTinte

SubCellular LocalizationCytoplasm

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (13)

Citations & References

Abstract

Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.

Authors:Hickman HD, Takeda K, Skon CN, Murray FR, Hensley SE, Loomis J, Barber GN, Bennink JR, Yewdell JW,

Journal:Nat Immunol

PubMed ID:18193049

'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More

Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.

Authors:Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK,

Journal:Science

PubMed ID:19325119

The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV ... More

New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ.

Authors:Dunn AK, Millikan DS, Adin DM, Bose JL, Stabb EV

Journal:Appl Environ Microbiol

PubMed ID:16391121

Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be ... More

A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells.

Authors:

Journal:Front Pharmacol

PubMed ID:32410987

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway.

Authors:

Journal:Oxid Med Cell Longev

PubMed ID:35237383

View all Sondas fluorescentes CellTracker™ citations