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CellTracker™ Fluoreszenzsonden
Zitierungen und Referenzen (13)
Invitrogen™

CellTracker™ Fluoreszenzsonden

Ein Fluoreszenzfarbstoff, mit dem sich die Bewegung und Position von Zellen ideal überwachen lässt
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KatalognummerMengeFarbstofftyp
C128815 mgCellTracker Blau CMF2HC
C21105 mgCellTracker™ Blau CMAC
C21115 mgCellTracker™ Blau CMHC
C3456520 x 15 μgAndere Etiketten oder Farbstoffe
C702520 x 50 μgCellTracker Grün CMFDA
C29251mgCellTracker Grün CMFDA
C21025 mgBODIPY Farbstoffe
C3455120 x 50 μgCellTracker Orange CMRA
C29271 mgCellTracker Orange CMTMR
C3455220 x 50 μgCellTracker™ Red CMTPX
C100945 × 0.1 mgThiolTracker™ Violett
Katalognummer C12881
Preis (EUR)
481,65
Exklusiv online
512,00
Ersparnis 30,35 (6%)
Each
Menge:
5 mg
Farbstofftyp:
CellTracker Blau CMF2HC
Preis (EUR)
481,65
Exklusiv online
512,00
Ersparnis 30,35 (6%)
Each
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Der Farbstoff wird gut beibehalten und ermöglicht so ein Tracking der zellulären Bewegungen über mehrere Generationen hinweg.
  • Die grünen Anregungs-/Emissionsspektren (Maxima bei 492/517 nm) sind optimal für das Multiplexing mit roten Fluoreszenzfarbstoffen und Proteinen.
  • Einfache Verwendung: Medium entfernen, Farbstoff hinzugeben, 30 Minuten inkubieren, Zellen anzeigen
  • Retention des Fluoreszenzsignals >72 Stunden (typischerweise drei bis sechs Generationen)
  • Geringe Zytotoxizität: Keine Auswirkungen auf Zellviabilität oder Proliferation
  • Konzipiert, um frei durch die Zellmembranen in die Zellen zu gelangen, wo er sich in nicht membrangängige Reaktionsprodukte verwandelt
  • Der Farbstoff wird an Tochterzellen übertragen, nicht jedoch an benachbarte Zellen in einer Population.
  • Stabil, ungiftig bei Arbeitskonzentrationen, guter Halt in Zellen und hell fluoreszierend bei physiologischem pH

Zelladhäsion, Zellanalyse, Zellproliferation, Zelltracing und -tracking, Zellviabilität und Zytotoxizität, Zellviabilität, -proliferation und -funktion, Zelluläre Bildgebung, Zelluläre Toxikologie-Assays, Chemotaxis und Zellmigration, Arzneimittelforschung und -entwicklung, allgemeines Zelltracing, Glutathion-Nachweis, High-Content Screening (HCS), Immunfluoreszenz (IF), Immunfluoreszenzfärbung und Nachweis, Ionische Homöostase und -signalisierung, Mikrobielles Tracking, Nitrooxidativer Stress, zielbasierte ADME/Tox-Assays, pH-Nachweis

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
FarbeBlau
BeschreibungCellTracker™ Blue CMF2HC Dye, 5 mg
FarbstofftypCellTracker Blau CMF2HC
Emission464
Anregungswellenlängenbereich371⁄464
FormDry Powder
ProduktlinieCellTracker
Menge5 mg
ReagenztypZell-Tracking-Verbindungen, Zell-Kennzeichnungsreagenzien
VersandbedingungRaumtemperatur, Raumtemperatur
MarkertypAndere Markierungen oder Farbstoffe
ProdukttypFarbstoff
SubCellular LocalizationCytoplasm
Unit SizeEach
Inhalt und Lagerung
Im Tiefkühlgerät (-5 bis -30 °C) lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellTracker™ Fluoreszenzsonden FAQs

Zitierungen und Referenzen (13)

Zitierungen und Referenzen
Abstract
Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
Authors:Hickman HD, Takeda K, Skon CN, Murray FR, Hensley SE, Loomis J, Barber GN, Bennink JR, Yewdell JW,
Journal:Nat Immunol
PubMed ID:18193049
'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.
Authors:Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK,
Journal:Science
PubMed ID:19325119
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV ... More
New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ.
Authors:Dunn AK, Millikan DS, Adin DM, Bose JL, Stabb EV
Journal:Appl Environ Microbiol
PubMed ID:16391121
Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be ... More
A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells.
Authors:
Journal:Front Pharmacol
PubMed ID:32410987
α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway.
Authors:
Journal:Oxid Med Cell Longev
PubMed ID:35237383
View all CellTracker™ Fluoreszenzsonden citations