CellTracker™ Blue CMF2HC (4-chloromethyl-6,8-difluoro-7-hydroxycoumarin) is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The blue excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.
• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells • Fluorescent signal retention of >72 hours (typically three to six generations) • Blue excitation/emission spectra (371/464 nm maxima) ideal for multiplexing • Low cytotoxicity—does not affect viability or proliferation
The CellTracker™ Blue CMF2HC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell-impermeant reaction products. CellTracker™ Blue CMF2HC dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Blue CMF2HC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Blue CMF2HC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.
For Research Use Only. Not for use in diagnostic procedures.