Thermo Scientific™

EZ-Link™ Biotin-PEG12-DBCO

Catalog number: C20042
Thermo Scientific™

EZ-Link™ Biotin-PEG12-DBCO

Catalog number: C20042
Catalog Number
C20042
Unit Size
10 mg
Price (USD)
Price: 242.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C2004210 mg
Price: 242.00
Your Price:
-
Product Overview
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EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach. The DBCO group reacts preferentially with an azide through a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form a stable triazole linkage with an azide-labeled coupling partner. EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and reach.

EZ-Link Biotin-PEG12-DBCO features include:
• Bioorthogonal—DBCO and azides react selectively to form triazole linkages
• Enhanced solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms irreversible and stable triazole linkage

Classic click reactions involve a copper-catalyzed azide-alkyne cyclo­addition to label or conjugate molecules. A drawback of this approach is that copper ions can impair protein function, harm cells, and reduce the fluorescence of fluorophores. DBCO is distinct in that it doesn’t require copper to catalyze the reaction with an azide for triazole formation. The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, preventing copper-induced damage to cells or proteins.

Specifications

Chemical Reactivity
Azide
Quantity
10 mg
Spacer Arm Length
46.3 Å
Crosslinker Type
Heterobifunctional
Labeling Method
Chemical Labeling
Reactive Moiety
DBCO, Biotin
Shipping Condition
Wet Ice
Product Line
EZ-Link™
Solubility
DMSO or DMF

Contents & Storage

Store desiccated at –20°C