CoroNa™ Green, AM, cell permeant - Special Packaging

Zitierungen und Referenzen (25)

Invitrogen™

CoroNa™ Green, AM, cell permeant - Special Packaging

CoroBa Green-Farbstoff ist ein Natriumionenindikator, der die grüne Fluoreszenz-Emissionsintensität nach Bindung von Na+-Ionen mit geringer Wellenlängenverschiebung erhöht. Wie unsere anderenWeitere Informationen

Katalognummer	Menge
C36676	20 x 50 μg

Katalognummer C36676

Preis (EUR)

1.079,65

Exklusiv online

1.136,00

Ersparnis 56,35 (5%)

Menge:

20 x 50 μg

Preis (EUR)

1.079,65

Exklusiv online

1.136,00

Ersparnis 56,35 (5%)

CoroBa Green-Farbstoff ist ein Natriumionenindikator, der die grüne Fluoreszenz-Emissionsintensität nach Bindung von Na⁺-Ionen mit geringer Wellenlängenverschiebung erhöht. Wie unsere anderen Natriumindikatoren, SBFI (Kat.-Nr. S1262, S1263, S1264) und Sodium Green-Farbstoff (Kat.-Nr. ermöglicht auch der CoroNa Green-Indikator die räumliche und zeitliche Auflösung von Na⁺-Konzentrationen bei Vorhandensein physiologischer Konzentrationen anderer monovalenter Kationen. CoroNa Green AM-Farbstoff diffundiert effizienter in Zellen als Sodium Green-Tetraacetat, und der CoroNa Green Indikator reagiert auf einen breiteren Na⁺-Konzentrationsbereich. Mit Absorptions-/Emissionsmaxima von ∼492/516 nm in der Form mit gebundenen Na⁺-Ionen ist der Nachweis von CoroNa Grün-Farbstoff mit allen Geräten möglich, die Fluorescein erkennen können. CoroNa Green-Farbstoff ist auch in nicht membrangängiger Form (Kat.-Nr. C36675) erhältlich.

Weitere Informationen zu Ionenindikatoren wie Calcium-, Kalium-, pH-Wert- und Membranpotentialindikatoren ›

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

NachweisverfahrenFluoreszent, Fluoreszent

FarbstofftypNatriumindikator

Menge20 x 50 μg

VersandbedingungRaumtemperatur, Raumtemperatur

Zur Verwendung mit (Anwendung)Viabilität und Proliferation von Zellen

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop

ProduktlinieCoroNa

ProdukttypNatriumionen-Indikator

Unit SizeEach

Inhalt und Lagerung

Im Tiefkühlgerät (-5 bis -30 °C) lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (25)

Zitierungen und Referenzen

Abstract

Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.

Authors:Zhang J, Liu H, Sun J, Li B, Zhu Q, Chen S, Zhang H,

Journal:PLoS One

PubMed ID:22279586

'Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ?-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma ... More

Diacylglycerol analogues activate second messenger-operated calcium channels exhibiting TRPC-like properties in cortical neurons.

Authors:Tu P, Kunert-Keil C, Lucke S, Brinkmeier H, Bouron A,

Journal:J Neurochem

PubMed ID:19094061

'The lipid diacylglycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was used to verify the existence of DAG-sensitive channels in cortical neurons dissociated from E13 mouse embryos. Calcium imaging experiments showed that OAG increased the cytosolic concentration of Ca(2+) ([Ca(2+)]i) in nearly 35% of the KCl-responsive cells. These Ca(2+) responses disappeared in a ... More

Membrane phosphatidylserine distribution as a non-apoptotic signalling mechanism in lymphocytes.

Authors:Elliott JI, Surprenant A, Marelli-Berg FM, Cooper JC, Cassady-Cain RL, Wooding C, Linton K, Alexander DR, Higgins CF

Journal:Nat Cell Biol

PubMed ID:16025105

'Phosphatidylserine (PS) exposure is normally associated with apoptosis and the removal of dying cells. We observed that PS is exposed constitutively at high levels on T lymphocytes that express low levels of the transmembrane tyrosine phosphatase CD45RB. CD45 was shown to be a negative regulator of PS translocation in response ... More

Loss of halophytism by interference with SOS1 expression.

Authors:Oh DH, Leidi E, Zhang Q, Hwang SM, Li Y, Quintero FJ, Jiang X, D'Urzo MP, Lee SY, Zhao Y, Bahk JD, Bressan RA, Yun DJ, Pardo JM, Bohnert HJ,

Journal:Plant Physiol

PubMed ID:19571313

'The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of ... More

Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.

Authors:Bortner CD, Sifre MI, Cidlowski JA,

Journal:J Biol Chem

PubMed ID:18187415

'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More

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