CoroNa™ Green, AM, cell permeant - Special Packaging

Citas y referencias (25)

Invitrogen™

CoroNa™ Green, AM, cell permeant - Special Packaging

El colorante CoroNa Green es un indicador de iones de sodio que muestra un aumento en la intensidad de emisiónMás información

Número de catálogo	Cantidad
C36676	20 x 50 μg

Número de catálogo C36676

Precio (MXN)

Cantidad:

20 x 50 μg

El colorante CoroNa Green es un indicador de iones de sodio que muestra un aumento en la intensidad de emisión de fluorescencia verde al unir Na⁺, con poco cambio en la longitud de onda. Al igual que nuestros otros indicadores de sodio, SBFI (n.º cat. S1262, S1263, S1264) y colorante verde sódico (n.º cat. S6900, S6901), el indicador CoroNa Green permite la resolución espacial y temporal de las concentraciones de Na⁺ en presencia de concentraciones fisiológicas de otros cationes monovalentes. El colorante CoroNa Green AM se carga en las células de manera más eficiente que el tetraacetato verde sódico, y el indicador de CoroNa Green responde a un rango más amplio de concentración de Na⁺ Con un máximo de absorción/emisión de ∼492/516 nm para forma unida a Na⁺, el colorante CoroNa Green puede ser detectado por cualquier instrumento que pueda detectar fluoresceína. El colorante CoroNa Green también está disponible en forma impenetrable en las células (n.º de cat. C36675).

Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Tipo de coloranteIndicador de sodio

Cantidad20 x 50 μg

Condiciones de envíoTemperatura ambiente

Para utilizar con (aplicación)Viabilidad y proliferación celulares

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosCoroNa

Tipo de productoIndicador de iones de sodio

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (25)

Citations & References

Abstract

Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.

Authors:Zhang J, Liu H, Sun J, Li B, Zhu Q, Chen S, Zhang H,

Journal:PLoS One

PubMed ID:22279586

'Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ?-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma ... More

Diacylglycerol analogues activate second messenger-operated calcium channels exhibiting TRPC-like properties in cortical neurons.

Authors:Tu P, Kunert-Keil C, Lucke S, Brinkmeier H, Bouron A,

Journal:J Neurochem

PubMed ID:19094061

'The lipid diacylglycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was used to verify the existence of DAG-sensitive channels in cortical neurons dissociated from E13 mouse embryos. Calcium imaging experiments showed that OAG increased the cytosolic concentration of Ca(2+) ([Ca(2+)]i) in nearly 35% of the KCl-responsive cells. These Ca(2+) responses disappeared in a ... More

Membrane phosphatidylserine distribution as a non-apoptotic signalling mechanism in lymphocytes.

Authors:Elliott JI, Surprenant A, Marelli-Berg FM, Cooper JC, Cassady-Cain RL, Wooding C, Linton K, Alexander DR, Higgins CF

Journal:Nat Cell Biol

PubMed ID:16025105

'Phosphatidylserine (PS) exposure is normally associated with apoptosis and the removal of dying cells. We observed that PS is exposed constitutively at high levels on T lymphocytes that express low levels of the transmembrane tyrosine phosphatase CD45RB. CD45 was shown to be a negative regulator of PS translocation in response ... More

Loss of halophytism by interference with SOS1 expression.

Authors:Oh DH, Leidi E, Zhang Q, Hwang SM, Li Y, Quintero FJ, Jiang X, D'Urzo MP, Lee SY, Zhao Y, Bahk JD, Bressan RA, Yun DJ, Pardo JM, Bohnert HJ,

Journal:Plant Physiol

PubMed ID:19571313

'The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of ... More

Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.

Authors:Bortner CD, Sifre MI, Cidlowski JA,

Journal:J Biol Chem

PubMed ID:18187415

'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More

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