Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.
AuthorsZhang J, Liu H, Sun J, Li B, Zhu Q, Chen S, Zhang H,
JournalPLoS One
PubMed ID22279586
'Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ?-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma ... More
Diacylglycerol analogues activate second messenger-operated calcium channels exhibiting TRPC-like properties in cortical neurons.
AuthorsTu P, Kunert-Keil C, Lucke S, Brinkmeier H, Bouron A,
JournalJ Neurochem
PubMed ID19094061
'The lipid diacylglycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was used to verify the existence of DAG-sensitive channels in cortical neurons dissociated from E13 mouse embryos. Calcium imaging experiments showed that OAG increased the cytosolic concentration of Ca(2+) ([Ca(2+)]i) in nearly 35% of the KCl-responsive cells. These Ca(2+) responses disappeared in a ... More
Membrane phosphatidylserine distribution as a non-apoptotic signalling mechanism in lymphocytes.
AuthorsElliott JI, Surprenant A, Marelli-Berg FM, Cooper JC, Cassady-Cain RL, Wooding C, Linton K, Alexander DR, Higgins CF
JournalNat Cell Biol
PubMed ID16025105
'Phosphatidylserine (PS) exposure is normally associated with apoptosis and the removal of dying cells. We observed that PS is exposed constitutively at high levels on T lymphocytes that express low levels of the transmembrane tyrosine phosphatase CD45RB. CD45 was shown to be a negative regulator of PS translocation in response ... More
Loss of halophytism by interference with SOS1 expression.
AuthorsOh DH, Leidi E, Zhang Q, Hwang SM, Li Y, Quintero FJ, Jiang X, D'Urzo MP, Lee SY, Zhao Y, Bahk JD, Bressan RA, Yun DJ, Pardo JM, Bohnert HJ,
JournalPlant Physiol
PubMed ID19571313
'The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of ... More
Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.
AuthorsBortner CD, Sifre MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID18187415
'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More
Induction of vertebrate regeneration by a transient sodium current.
AuthorsTseng AS, Beane WS, Lemire JM, Masi A, Levin M,
JournalJ Neurosci
PubMed ID20881138
'Amphibians such as frogs can restore lost organs during development, including the lens and tail. To design biomedical therapies for organ repair, it is necessary to develop a detailed understanding of natural regeneration. Recently, ion transport has been implicated as a functional regulator of regeneration. Whereas voltage-gated sodium channels play ... More
Fluorescent metal ion indicators based on benzoannelated crown systems: a green fluorescent indicator for intracellular sodium ions.
AuthorsMartin VV, Rothe A, Gee KR
JournalBioorg Med Chem Lett
PubMed ID15780620
The synthesis and metal binding properties of cation-sensitive fluorescent indicators intended for biological applications are described. The increase of the crown ether ring size enhances the affinity for larger cations, but weakens the fluorescent response and selectivity. A compound having a 15-crown-5 chelator directly attached to a 2,7-difluoroxanthenone fluorophore loads ... More
Rice cultivars with differing salt tolerance contain similar cation channels in their root cells.
AuthorsKavitha PG, Miller AJ, Mathew MK, Maathuis FJ,
JournalJ Exp Bot
PubMed ID22345644
Salinity poses a major threat for agriculture worldwide. Rice is one of the major crops where most of the high-yielding cultivars are highly sensitive to salinity. Several studies on the genetic variability across rice cultivars suggest that the activity and composition of root plasma membrane transporters could underlie the observed ... More
AuthorsOki K, Plonczynski MW, Luis Lam M, Gomez-Sanchez EP, Gomez-Sanchez CE,
JournalEndocrinology
PubMed ID22315453
Primary aldosteronism is the most common cause of secondary hypertension, most frequently due to an aldosterone-producing adenoma or idiopathic hyperaldosteronism. Somatic mutations of the potassium channel KCNJ5 in the region of the selectivity filter have been found in a significant number of aldosterone-producing adenomas. There are also familial forms of ... More
AuthorsPoburko D, Liao CH, Lemos VS, Lin E, Maruyama Y, Cole WC, van Breemen C,
JournalCirc Res
PubMed ID17872462
The Na+/Ca2+ exchanger (NCX) is increasingly recognized as a physiological mediator of Ca2+ influx and significantly contributes to salt-sensitive hypertension. We recently reported that Ca2+ influx by the NCX (1) is the primary mechanism of Ca2+ entry in purinergically stimulated rat aorta smooth muscle cells and (2) requires functional coupling ... More
The cellular mechanisms of neuronal swelling underlying cytotoxic edema.
AuthorsRungta RL, Choi HB, Tyson JR, Malik A, Dissing-Olesen L, Lin PJ, Cain SM, Cullis PR, Snutch TP, MacVicar BA,
Journal
PubMed ID25910210
Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular Cl(-) that is required for ... More
Remote excitation of neuronal circuits using low-intensity, low-frequency ultrasound.
AuthorsTyler WJ, Tufail Y, Finsterwald M, Tauchmann ML, Olson EJ, Majestic C,
JournalPLoS ONE
PubMed ID18958151
Possessing the ability to noninvasively elicit brain circuit activity yields immense experimental and therapeutic power. Most currently employed neurostimulation methods rely on the somewhat invasive use of stimulating electrodes or photon-emitting devices. Due to its ability to noninvasively propagate through bone and other tissues in a focused manner, the implementation ... More
Na+-dependent HCO3- import by the slc4a10 gene product involves Cl- export.
AuthorsDamkier HH, Aalkjaer C, Praetorius J,
JournalJ Biol Chem
PubMed ID20566632
The slc4a10 gene encodes an electroneutral Na(+)-dependent HCO(3)(-) importer for which the precise mode of action remains unsettled. To resolve this issue, intracellular pH (pH(i)) recordings were performed upon acidification in the presence of CO(2)/HCO(3)(-) by 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorometry of stably slc4a10-transfected NIH-3T3 fibroblasts. slc4a10 expression induced a significant Na(+)-dependent ... More
Novel Gating and Sensitizing Mechanism of Capsaicin Receptor (TRPV1): TONIC INHIBITORY REGULATION OF EXTRACELLULAR SODIUM THROUGH THE EXTERNAL PROTONATION SITES ON TRPV1.
AuthorsOhta T, Imagawa T, Ito S,
JournalJ Biol Chem
PubMed ID18230619
Transient receptor potential V1 (TRPV1) is a nonselective cation channel expressed in nociceptors and activated by capsaicin. TRPV1 detects diverse stimuli, including acid, heat, and endogenous vanilloids, and functions as a molecular integrator of pain perception. Herein we demonstrate a novel regulatory role of extracellular Na(+) ([Na(+)](o)) on TRPV1 function. ... More
Properties of the new fluorescent Na+ indicator CoroNa Green: comparison with SBFI and confocal Na+ imaging.
AuthorsMeier SD, Kovalchuk Y, Rose CR
JournalJ Neurosci Methods
PubMed ID16488020
Neuronal activity causes substantial Na+ transients in fine cellular processes such as dendrites and spines. The physiological consequences of such Na+ transients are still largely unknown. High-resolution Na+ imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na+ indicator sodium-binding benzofuran isophthalate (SBFI) ... More
TRPV4 antagonists ameliorate ventriculomegaly in a rat model of hydrocephalus.
Authors
JournalJCI Insight
PubMed ID32938829
Variation in dietary salt intake induces coordinated dynamics of monocyte subsets and monocyte-platelet aggregates in humans: implications in end organ inflammation.
Authors
JournalPLoS One
PubMed ID23593194
Na+ extrusion from the cytosol and tissue-specific Na+ sequestration in roots confer differential salt stress tolerance between durum and bread wheat.
Authors
JournalJ Exp Bot
PubMed ID29897491
Membrane trauma and Na+ leak from Nav1.6 channels.
Authors
JournalAm J Physiol Cell Physiol
PubMed ID19657055
Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines.
Authors
JournalEur Biophys J
PubMed ID27660079
Bacterial Intracellular Sodium Ion Measurement using CoroNa Green.
Authors
JournalBio Protoc
PubMed ID34458422
Quantitative determination of cellular [Na+] by fluorescence lifetime imaging with CoroNaGreen.
Authors
JournalJ Gen Physiol
PubMed ID31597684
Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress.
Authors
JournalPlant Direct
PubMed ID34027297
Mechanism of pyrethroid pesticide-induced apoptosis: role of calpain and the ER stress pathway.
Authors
JournalToxicol Sci
PubMed ID21555338
Changes in transepithelial electrical resistance and intracellular ion concentration in TGF-β-induced epithelial-mesenchymal transition of retinal pigment epithelial cells.