MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells

Description

MultiShot FlexPlate OmniMAX 2 T1^R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Abundance of colonies with OmniMAX 2 T1^R cloning capabilities
MultiShot FlexPlate OmniMAX 2 T1^R cells are an improved chemically competent E. coli cell line, perfect for use in all cloning applications, including Gateway cloning, and provide transformation efficiencies in the MultiShot FlexPlate format of >5 x 10⁸ cfu/µg with control plasmid DNA. These cells also provide efficient transformation of highly methylated DNA, since OmniMAX 2 T1^R cells lack the E. coli K-12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)).

Key features of the MultiShot FlexPlate OmniMAX 2 T1^R Competent Cells include:
• Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene—ideal for transformation of Gateway and TOPO reactions
• mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Highest transformation efficiency in FlexWell format (>5x10⁸ cfu/µg control DNA)
• endA1 for increased plasmid yield and quantity
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

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Specifications

Bacterial or Yeast Strain:	OmniMAX™2
Blue/White Screening:	Yes
Cloning Methylated DNA:	Yes
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Contains F' Episome
High-throughput Compatibility:	High-throughput Compatible
Plasmid:	May be used for plasmids > 20 kb
Product Line:	MultiShot™, OmniMAX™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Shipping Condition:	Dry Ice
Format:	96-well plate
Species:	E. coli

Contents & storage