Choose the right electroporation cuvette for your strain. Available in a variety of sizes.
Learn more about Electroporation Cuvettes
Electrocompetent Cells
Electrocompetent cells work using the electroporation process. Electrical pulses created pores that allows genetic material to permeate the bacterial membrane. Invitrogen offers a variety of electrocompetent E. coli cells to reliably clone your DNA with high efficiency. The convenient packaging formats and a variety of electroporation cuvettes will fulfill all of your electrocompetent cell transformation requirements.
Electroporation Cuvettes
Electrocompetent cells available
Cell Strain |
Description | Application | Transformation Efficiency | |
|---|---|---|---|---|
MegaX DH10B T1R Electrocomp Cells  |
MegaX DH10B T1R Electrocomp cells are the highest-efficiency electrocompetent cells. See additional details below. |
Ideal for highly demanding cloning and library construction applications | >3 x 1010 cfu/μg | Order Now |
| ElectroMAX DH10BT 1 R Cells |
High efficiency cells derived from DH10Bstrain. tonA genotype for T1 and T5 phage resistance. | Efficient cloning of prokaryotic and eukaryotic genomic DNA. Efficient plasmid rescue from eukaryotic genomes. | >1 × 1010 cfu/μg | Order Now |
| ElectroMAX DH10B Cells |
Derived from the DH10B strain in a high efficiency electrocompetent format. Also available in bulk format. | Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. | >1 × 1010 cfu/μg | Order Now |
| ElectroMAX DH5α-E Cells | Derived from the DH5α strain. Suitable for transformation by electroporation. | Procedures requiring high transformation efficiencies, such as cDNA library generation or with limited input DNA. | >1 × 1010 cfu/μg | Order Now |
| One Shot TOP10 Electrocompetent Cells | Genetically similar to the DH10B strain. Available in single-use One Shot format. | Used for general cloning applications. Found in many TOPO cloning and expression kits. | >1 × 1010 cfu/μg | Order Now |
| TOP10 Electrocompetent Cells | Genetically similar to the reliable DH10B strain. | Used for general cloning applications. Found in many TOPO cloning and expression kits. | >1 × 1010 cfu/μg | Order Now |
| TOP10F’ Electrocompetent Cells | Genetically similar to the reliable DH10B strain with the F’ genotype. | Used for general cloning applications. | >1 × 1010 cfu/μg | Order Now |
| ElectroMAX DH12S Cells | A derivative of DH10B are suitable for transformation by electroporation. | A unique genotype to support production of exceptionally clean ssDNA. | >1 × 1010 cfu/μg | Order Now |
| ElectroMAX Stbl4 Competent Cells | Suitable for transformation by electroporation. Specifically designed for cloning unstable inserts. | >5 × 109 cfu/μg | Order Now |
Highest-efficiency electrocompetent cells
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available (Figure 1), with a guaranteed three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC control DNA). They are ideal for highly demanding cloning and library construction applications. MegaX cells have the same genotype as the widely used DH10B™ T1R strain, including tonA to prevent T1 and T5 lytic phage infection and safeguard your valuable clones and libraries; however, they are manufactured using an improved process that results in a significantly higher transformation efficiency. Available in the bulk Electrocomp format.
Figure 1. MegaX DH10B T1R Electrocomp cells consistently outperform the competition. L: E.cloni® 10G SUPREME cells (Lucigen); G:GC10™ Thunderbolt™ cells (GeneChoice); S: ElectroTen-Blue® electroporation-competent cells (Stratagene).
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.