Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

Catalog number:  C640003

Related applications:

Transformation

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Description

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.

Using MegaX DH10B T1R cells
MegaX DH10B T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)). The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH10B
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Electrocompetent
F'episome (to make ssDNA): Lacks F' Episome
Format: Tube(s)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid: May be used for plasmids > 100 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH10B, Electrocomp™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
Species: E. coli
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 3x10^10
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)

Contents & storage

Contains:
• MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• Recovery Medium: 3 bottles, 10 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .