Invitrogen™

MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Catalog number:  C869601

Related applications:

Transformation

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Description

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Mach1 T1R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1R cloning capabilities
MultiShot FlexPlate Mach1 T1R Competent Cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1R Competent Cells include:
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1398 for reduced occurrence of non-specific recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
tonA (fhuA) for T1 and T5 phage resistance
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and preventing contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increased productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: Mach1™
Blue/White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
Fast Growth: Yes
High-throughput Compatibility: High-throughput Compatible
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: Mach1™, MultiShot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 1x10^9
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Format: MultiShot StripWell
Improves Plasmid Quality: Yes
Species: E. coli
Quantity: 1 plate

Contents & storage

Contains:
• Mach1™ T1 Phage-Resistant (T1R) Chemically Competent E. coli: One 96-stripwell plate, 50 µl per well
• pUC19 DNA (10 pg/µl): 1 vials, 50 µl
• S.O.C. medium: 2 bottles, 15 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.