MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Catalog number: C869601

Invitrogen™  Related applications: Transformation

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MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments. The fast-growing Mach1™ T1R strain:

• Minimizes nonspecific recombination in cloned DNA (recA)
• Offers resistance to T1 and T5 phage (tonA)
• Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
• Allows blue/white screening of recombinant clones (lacZΔM15)

Two formats to choose from
• The convenient One Shot® format provides single-use, 50-µL aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate, which allows you to perform as few or as many transformations as you need without wasting reagents

Contents & storage

• Mach1™ T1 Phage-Resistant (T1R) Chemically Competent E. coli: One 96-stripwell plate, 50 µl per well
• pUC19 DNA (10 pg/µl): 1 vials, 50 µl
• S.O.C. medium: 2 bottles, 15 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.


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