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OriGene
SPN Monoclonal Antibody (OTI2D4), TrueMAB™
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FIGURE: 1 / 1
SPN Antibody (CF800786) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPN (RC204195, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SPN. Positive lysates LY418888 (100 µg) and LC418888 (20 µg) can be purchased separately from OriGene.
Product Details
CF800786
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2D4
Immunogen
Full length human recombinant protein of human SPN produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115; sialophorin precursor; unnamed protein product
Gene Aliases: CD43; GALGP; GPL115; LEU-22; LSN; SPN
UniProt ID: (Human) P16150
Entrez Gene ID: (Human) 6693
response to protozoan
negative regulation of type IV hypersensitivity
T-helper 1 cell lineage commitment
chemotaxis
immune response
cellular defense response
negative regulation of cell adhesion
establishment or maintenance of cell polarity
signal transduction
cell surface receptor signaling pathway
T cell costimulation
positive regulation of tumor necrosis factor production
T cell proliferation
positive regulation of T cell proliferation
negative regulation of T cell proliferation
defense response to bacterium
negative thymic T cell selection
regulation of defense response to virus
regulation of immune response
regulation of T cell activation
negative regulation of T cell activation
leukocyte tethering or rolling
apoptotic signaling pathway
regulation of T cell migration
positive regulation of T cell migration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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