Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae.
AuthorsLevashina EA, Moita LF, Blandin S, Vriend G, Lagueux M, Kafatos FC
JournalCell
PubMed ID11257225
'We characterize a novel hemocyte-specific acute phase glycoprotein from the malaria vector, Anopheles gambiae. It shows substantial structural and functional similarities, including the highly conserved thioester motif, to both a central component of mammalian complement system, factor C3, and to a pan-protease inhibitor, alpha2-macroglobulin. Most importantly, this protein serves as ... More
Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.
AuthorsCarrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,
JournalJ Immunol
PubMed ID17548620
'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More
Methodological aspects of assessing phagocytosis of Vibrio anguillarum by leucocytes of gilthead seabream (Sparus aurata L.) by flow cytometry and electron microscopy.
AuthorsEsteban MA, Mulero V, Muñoz J, Meseguer J
JournalCell Tissue Res
PubMed ID9634605
'In this paper we optimize a flow cytometric method for evaluating the phagocytic activity of leucocytes in gilthead seabream (Sparus aurata L.) and characterize the phagocytic cells observed. Optimal conditions were established for the fluorescein-labelling and analysis of the bacterium Vibrio anguillarum by flow cytometry. Head-kidney leucocytes were incubated with ... More
Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic.
AuthorsBaorto DM, Gao Z, Malaviya R, Dustin ML, van der Merwe A, Lublin DM, Abraham SN
JournalNature
PubMed ID9335508
'Strains of Escherichia coli persist within the human gut as normal commensals, but are frequent pathogens and can cause recurrent infection. Here we show that, in contrast to E. coli subjected to opsonic interactions stimulated by the host''s immune response, E. coli that bind to the macrophage surface exclusively through ... More
Comparative in vitro investigations of the influence of mofebutazone, phenylbutazone and diclofenac on phagocytosis and respiratory burst of human peripheral blood leucocytes.
AuthorsNeumüller J, Tohidast-Akrad M
JournalArzneimittelforschung
PubMed ID8024638
'The phagocytosis and the release of oxidative products generated by the respiratory burst have been studied under the influence of the non-steroidal anti-inflammatories (NSAID) phenylbutazone (PB), mofebutazone (monophenylbutazone, MPB) and diclofenac (DF) using phagocytes of the peripheral blood from healthy human donors and patients with soft tissue rheumatism. The measurement ... More
Involvement of CD44 and the cytoskeletal linker protein ankyrin in human neutrophil bacterial phagocytosis.
AuthorsMoffat FL, Han T, Li ZM, Peck MD, Falk RE, Spalding PB, Jy W, Ahn YS, Chu AJ, Bourguignon LY
JournalJ Cell Physiol
PubMed ID8816918
'The leukocyte CD44 and CD45 cell surface receptors are associated via the linker proteins ankyrin and fodrin with the cytoskeleton, which itself is important in immune cell functions such as adherence, chemotaxis, and phagocytosis. The effects of rat antihuman CD44 and CD45 monoclonal antibodies on phagocytosis of fluoresceinated heat-killed Staphylococcus ... More
Increase in phagocytosis after geldanamycin treatment or heat shock: role of heat shock proteins.
AuthorsVega VL, De Maio A
JournalJ Immunol
PubMed ID16210633
'The response to injury is activated at the systemic and cellular levels. At the systemic level, phagocytosis plays a key role in controlling infections and clearing necrotic and apoptotic cells. The expression of heat shock proteins (Hsp), which is a well-conserved process, is a major component of cellular response to ... More
A phage display screen and binding studies with acetylated low density lipoprotein provide evidence for the importance of the scavenger receptor cysteine-rich (SRCR) domain in the ligand-binding function of MARCO.
AuthorsChen Y, Sankala M, Ojala JR, Sun Y, Tuuttila A, Isenman DE, Tryggvason K, Pikkarainen T
JournalJ Biol Chem
PubMed ID16524885
'MARCO is a class A scavenger receptor capable of binding both gram-negative and -positive bacteria. Using the surface plasmon resonance technique, we show here that a recombinant, soluble form of MARCO, sMARCO, binds the major gram-negative and -positive bacterial surface components, lipopolysaccharide and lipoteichoic acid. Yet, the interaction of these ... More
Budesonide but not terbutaline decreases phagocytosis in alveolar macrophages.
AuthorsZetterlund A, Larsson PH, Müller-Suur C, Palmberg L, Larsson K
JournalRespir Med
PubMed ID9616506
'Alveolar macrophages are the most common cells in bronchoalveolar lavage fluid. The macrophages participate in the inflammatory response and defence of the airways by secretion of mediators and by phagocytizing foreign particles such as bacteria and viruses. beta-Agonists and glucocorticosteroids are the most frequently used drugs in asthma. Alveolar macrophages ... More
Opsonic antibodies to outer membrane protein P2 of nonencapsulated Haemophilus influenza are strain specific.
AuthorsTroelstra A, Vogel L, van Alphen L, Eijk P, Jansen H, Dankert J
JournalInfect Immun
PubMed ID8112849
'The ability of monoclonal antibodies (MAbs) specific for variable and conserved epitopes of outer membrane protein (OMP) P2 (b,c) of nonencapsulated Haemophilus influenza to promote opsonophagocytosis of this bacterium by human polymorphonuclear leucocytes (PMNs) was determined by flow cytometry. MAbs rendering PMNs fluorescent because of association with fluorescein isothiocyanate-labelled bacteria ... More
Differential involvement of Src family kinases in Fc gamma receptor-mediated phagocytosis.
AuthorsSuzuki T, Kono H, Hirose N, Okada M, Yamamoto T, Yamamoto K, Honda Z
JournalJ Immunol
PubMed ID10861086
'The tyrosine phosphorylation cascade originated from Fc gamma receptors (Fc gamma Rs) is essential for macrophage functions including phagocytosis. Although the initial step is ascribed to Src family tyrosine kinases, the role of individual kinases in phagocytosis signaling is still to be determined. In reconstitution experiments, we first showed that ... More
Effect of radiographic contrast media on granulocyte phagocytosis of Escherichia coli in a whole blood flow cytometric assay.
AuthorsLillevang ST, Albertsen M, Rasmussen F, Georgsen J, Egund N
JournalInvest Radiol
PubMed ID8144341
'RATIONALE AND OBJECTIVES. Earlier studies have demonstrated an adverse effect of radiographic contrast media (CM) on granulocyte phagocytosis. Most studies in the past have depended on granulocyte separative procedures that may themselves affect granulocyte functions. This study was performed to evaluate the effect of CM on phagocytosis using a flow ... More
Phagocytosis by human leukocytes, phagosomal pH and degradation of seven species of bacteria measured by flow cytometry.
AuthorsBassøe CF, Bjerknes R
JournalJ Med Microbiol
PubMed ID3968704
'Phagocytosis by human leukocytes, phagosomal pH and degradation of seven species of bacteria were studied by a flow cytometric method. The percentage of phagocytosing leukocytes was similar for all bacterial strains examined, but Salmonella typhi and Neisseria meningitidis were more slowly phagocytosed than other bacteria. The phagosomal pH surrounding the ... More
Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.
AuthorsSever-Chroneos Z, Krupa A, Davis J, Hasan M, Yang CH, Szeliga J, Herrmann M, Hussain M, Geisbrecht BV, Kobzik L, Chroneos ZC,
JournalJ Biol Chem
PubMed ID21123169
Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates ... More
Polymorphonuclear leucocyte dysregulation in patients with gram-negative septicaemia assessed by flow cytometry.
AuthorsWenisch C, Parschalk P, Hasenhündl M, Griesmacher A, Graninger W
JournalEur J Clin Invest
PubMed ID7656920
Flow cytometry was used to study phagocytic function and release of reactive oxygen intermediates (ROI) following phagocytosis by granulocytes in 14 patients (six female, eight male) with gram-negative septicaemia prior to, during, and after therapy compared with a group of healthy controls. Phagocytic capacity was assessed by measuring uptake of ... More
Structure of the human macrophage MARCO receptor and characterization of its bacteria-binding region.
AuthorsElomaa O, Sankala M, Pikkarainen T, Bergmann U, Tuuttila A, Raatikainen-Ahokas A, Sariola H, Tryggvason K
JournalJ Biol Chem
PubMed ID9468508
The primary structure of human macrophage receptor with collagenous structure (MARCO) was determined from cDNA clones and shown to be highly similar to that of mouse (Elomaa, O., Kangas, M., Sahlberg, C. , Tuukkanen, J., Sormunen, R., Liakka, A., Thesleff, I., Kraal, G., and Tryggvason, K. (1995) Cell 80, 603-609). ... More
Fungal peptide Destruxin A plays a specific role in suppressing the innate immune response in Drosophila melanogaster.
AuthorsPal S, St Leger RJ, Wu LP
JournalJ Biol Chem
PubMed ID17227774
Destruxins are a class of insecticidal, anti-viral, and phytotoxic cyclic depsipeptides that are also studied for their toxicity to cancer cells. They are produced by various fungi, and a direct relationship has been established between Destruxin production and the virulence of the entomopathogen Metarhizium anisopliae. Aside from opening calcium channels, ... More
Effect of cefodizime and ceftriaxone on phagocytic function in patients with severe infections.
AuthorsWenisch C, Parschalk B, Hasenhündl M, Wiesinger E, Graninger W
JournalAntimicrob Agents Chemother
PubMed ID7793871
Thirty patients with severe bacterial infections were treated with 50 mg of cefodizime per kg of body weight once daily or 50 mg of ceftriaxone per kg once daily for 10 +/- 3 days. The effect of cefodizime and ceftriaxone on the phagocytic capacity and generation of reactive oxygen intermediates ... More
A new simple fluorometric assay for phagocytosis.
AuthorsOda T, Maeda H
JournalJ Immunol Methods
PubMed ID3514764
A highly sensitive, but simple and quantitative, fluorometric assay method for phagocytosis by cells such as macrophages and polymorphonuclear leukocytes was developed by utilizing fluorescent particles. Escherichia coli, Serratia marcescens, yeast, and latex particles were conjugated with fluorescein isothiocyanate and used as fluorescent particles. The assay procedure requires phagocytic cells, ... More
CHARACTERIZATION OF MACROPHAGES AND NEUTROPHILIC GRANULOCYTES FROM THE PRONEPHROS OF CARP (CYPRINUS CARPIO)
AuthorsKemenade B, Groeneveld A, Rens B, Rombout J
JournalJ Exp Biol
PubMed ID9317515
To analyse the functional activity of different leucocyte types, carp pronephros cells were separated on Percoll density gradients and by use of fluorescence-activated cell sorting. Cell populations were characterised by light and electron microscopy and by flow cytometry. Fractions enriched in macrophages and neutrophilic granulocytes were subsequently analysed for phagocytic ... More
The peptidoglycan recognition protein PGRP-SC1a is essential for Toll signaling and phagocytosis of Staphylococcus aureus in Drosophila.
AuthorsGarver LS, Wu J, Wu LP
JournalProc Natl Acad Sci U S A
PubMed ID16407137
From a forward genetic screen for phagocytosis mutants in Drosophila melanogaster, we identified a mutation that affects peptidoglycan recognition protein (PGRP) SC1a and impairs the ability to phagocytose the bacteria Staphylococcus aureus, but not Escherichia coli and Bacillus subtilis. Because of the differences in peptidoglycan peptide linkages in these bacteria, ... More
Double fluorescent flow cytometric assessment of bacterial internalization and binding by epithelial cells.
This study describes a new flow cytometric method for assessment of phagocytosis of specific bacteria (bacillus Calmette-Guérin (BCG) and Escherichia coli) by bladder epithelial cells. The internalization assay consisted of labeling bacteria chemically with fluorescein isothiocyanate (FITC). Subsequent to incubation of fluoresceinated bacteria with internalizing cells, adherent nonphagocytosed bacteria were ... More
The influence of growth hormone on tumour necrosis factor and neutrophil leukocyte function in sepsis.
AuthorsBalteskard L, Unneberg K, Halvorsen D, Ytrebø LM, Waage A, Sjursen H, Revhaug A
JournalScand J Infect Dis
PubMed ID9360256
The aim of this study was to assess the influence of growth hormone (GH) in sepsis on the immune system represented by the circulating TNF-levels and the neutrophil leukocytes phagocytic capacity and respiratory burst, 22 piglets were randomized to 3 groups; pretreatment with GH (16 i.u.) before sepsis (n = ... More
A rapid and simple microfluorometric phagocytosis assay.
AuthorsWan CP, Park CS, Lau BH
JournalJ Immunol Methods
PubMed ID8509646
A microfluorometric method for phagocytosis study has been developed using fluorescein conjugated Escherichia coli K-12 particles. This technique is based on the uptake of fluorescent particles and quenching of extracellular fluorescence at the end of the assay. A murine macrophage cell line, J774, was used as a phagocyte model. The ... More
Monocyte differentiation up-regulates the expression of the lysosomal sialidase, Neu1, and triggers its targeting to the plasma membrane via major histocompatibility complex class II-positive compartments.
AuthorsLiang F, Seyrantepe V, Landry K, Ahmad R, Ahmad A, Stamatos NM, Pshezhetsky AV
JournalJ Biol Chem
PubMed ID16835219
Human sialidase (neuraminidase) Neu1 catalyzes lysosomal catabolism of sialylated glycoconjugates. Here we show that during the differentiation of monocytes and the monocytic cell line, THP-1, into macrophages, the majority of Neu1 relocalizes from the lysosomes to the cell surface. In contrast to other cellular sialidases Neu2, Neu3, and Neu4, whose ... More
Depressed phagocytosis and oxidative burst in polymorphonuclear leukocytes from individuals with pulmonary tuberculosis with or without human immunodeficiency virus type 1 infection.
AuthorsShalekoff S, Tiemessen CT, Gray CM, Martin DJ
JournalClin Diagn Lab Immunol
PubMed ID9455878
Phagocytosis and oxidative burst in whole-blood granulocytes were assessed by flow cytometry with Phagotest and Bursttest kits, respectively. Seventy individuals were included in this study: 15 healthy, normal donors, 18 human immunodeficiency virus (HIV) type 1 (HIV-1)-seropositive patients, 19 patients with pulmonary tuberculosis (TB), and 18 patients co-infected with Mycobacterium ... More
Generation and characterization of Dictyostelium cells deficient in a myosin I heavy chain isoform.
AuthorsJung G, Hammer JA
JournalJ Cell Biol
PubMed ID2141028
Motile activities such as chemotaxis and phagocytosis, which occur in Dictyostelium cells lacking myosin II, may be dependent upon myosin I. To begin to explore this possibility, we have engineered a disruption of the Dictyostelium myosin I heavy chain (DMIHC) gene described recently (Jung, G., C. L. Saxe III, A. ... More
The small Mr Ras-like GTPase Rap1 and the phospholipase C pathway act to regulate phagocytosis in Dictyostelium discoideum.
The function of the small-Mr Ras-like GTPase Rap1 remains largely unknown, but this protein has been demonstrated to regulate cortical actin-based morphologic changes in Dictyostelium and the oxidative burst in mammalian neutrophils. To test whether Rap1 regulates phagocytosis, we biochemically analyzed cell lines that conditionally and modestly overexpressed wild-type [Rap1 ... More
Analysis of CD44-containing lipid rafts: Recruitment of annexin II and stabilization by the actin cytoskeleton.
AuthorsOliferenko S, Paiha K, Harder T, Gerke V, Schwärzler C, Schwarz H, Beug H, Günthert U, Huber LA
JournalJ Cell Biol
PubMed ID10459018
CD44, the major cell surface receptor for hyaluronic acid (HA), was shown to localize to detergent-resistant cholesterol-rich microdomains, called lipid rafts, in fibroblasts and blood cells. Here, we have investigated the molecular environment of CD44 within the plane of the basolateral membrane of polarized mammary epithelial cells. We show that ... More