FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
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FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Thermo Scientific™

FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. ThisRead more
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Catalog NumberQuantity
EF06511000 U
EF06525 x 1000 U
EF0654300 U
Catalog number EF0651
Price (USD)/ 1,000 units
85.65
Online exclusive
101.00 
Save 15.35 (15%)
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Add to cart
Quantity:
1000 U
Request bulk or custom format
Price (USD)/ 1,000 units
85.65
Online exclusive
101.00 
Save 15.35 (15%)
Add to cart
FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalog numberEF0651
Price (USD)/ 1,000 units
85.65
Online exclusive
101.00 
Save 15.35 (15%)
-
Add to cart
Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C (see Figure 1 in Supporting Data). Therefore, removal of alkaline phosphatase is not required prior to ligation.

Highlights

Recombinant enzyme
Fast dephosphorylation—10 minutes at 37°C
Fast and complete inactivation—5 minutes at 75°C
Simultaneous digestion and dephosphorylation of vector DNA
100% active in restriction enzyme and PCR buffers
PCR clean-up in conjunction with Exo I
Protein dephosphorylation

One protocol for all types of DNA ends:
• 5'-overhangs
• 3'-overhangs
• blunt-ends
• single nucleotides

Applications

• Dephosphorylation of cloning vector DNA to prevent recircularization during ligation
• Simultaneous digestion and dephosphorylation of vector DNA
• PCR product clean-up: nucleotide degradation prior to sequencing of PCR product
• Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase
• Other applications where dephosphorylation of DNA and RNA substrates is necessary
• Protein dephosphorylation

Note

• Binding of FastAP Thermosensitive Alkaline Phosphatase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 65°C for 10 minutes, and chill on ice prior to electrophoresis.
• FastAP Thermosensitive Alkaline Phosphatase is active in all restriction enzyme buffers and may be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 U/μL
EnzymePhosphatase
Compatible BufferPCR Buffer, Enzyme Buffer
Quantity1000 U
Product TypeAlkaline Phosphatase
Unit Size1,000 units