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T4 DNA Ligase, HC (30 U/μL) - FAQs

View additional product information for T4 DNA Ligase, HC (30 U/μL) - FAQs (EL0013)

3 product FAQs found

What are white precipitates in the Thermo Scientific 10X T4 DNA Ligase Buffer that appear upon thawing?

The white precipitates are DTT in the buffer which may appear after freezing. To redissolve DTT, warm up the buffer to 37 degrees C by holding the vial between your palms, or incubating in a water bath for a few minutes, and mix well. The formation of DTT precipitates does not influence the quality of the buffer. The 10X T4 DNA Ligase Buffer is also available separately (Cat. No. B69).

What is the difference between Rapid DNA Ligation Kit and Thermo Scientific T4 DNA ligase with a standard buffer?

The speed of ligation is the main difference between the two. The Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature. The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation.

Why do I see differences in the units of T4 DNA ligase from Thermo Scientific and other vendors?

Thermo Scientific T4 DNA ligase activity is expressed in Weiss units. One Weiss unit is equivalent to approximately 200 cohesive end units (CEU) which may be used by other vendors. One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37 degrees C. One CEU is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of lambda DNA in 30 min at 16 degrees C.