Thermo Scientific™

T4 DNA Ligase (5 U/µL)

Catalog number: EL0014
Thermo Scientific™

T4 DNA Ligase (5 U/µL)

Catalog number: EL0014
Catalog Number
EL0014
Unit Size
200 units
Price (USD)
73.75
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
236.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 1,000 units
Price (USD)
866.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EL0014200 units
73.75
Your Price:
***
EL00111,000 units
236.00
Your Price:
***
EL00125 x 1,000 units
866.00
Your Price:
***
Product Overview
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Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast—sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligation

Applications

• Cloning of restriction enzyme generated DNA fragments
• Cloning of PCR products
• Joining of double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Amplified fragment length polymorphism (AFLP)
• Ligase-mediated RNA detection (see Reference 3)
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Self-circularization of linear DNA.

Includes

• T4 DNA Ligase
• 10X T4 DNA Ligase Buffer
• 50% PEG Solution

Notes

• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
• The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
• Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.

Specifications

Concentration
5 U/μL
Enzyme
DNA Ligase
Compatible Buffer
10X T4 DNA Ligase Buffer
Quantity
200 U
Product Type
T4 DNA Ligase

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