Thermo Scientific ATP (adenosine 5'-triphosphate), Tris buffered is an extremely stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with Tris base. The nucleotide has greater than 99% purity, is free of nuclease activities and functionally tested in in vitro transcription.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of trace contaminating nucleotides, endo-, exodeoxyribonuclease and ribonuclease activities
• Higher in vitro transcription yields using Tris buffered NTPs in comparison to NTPs, titrated with NaOH
• High stability—stable for 3 years at -20°C and after multiple freeze-thaw cycles

Applications

• in vitro transcription
• RNA amplification
• siRNA synthesis
• aRNA synthesis
• Ligation
• Phosphorylation