S1 Nuclease (100 U/μL)
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S1 Nuclease (100 U/μL)
Thermo Scientific™

S1 Nuclease (100 U/μL)

Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is five times more active onRead more
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Catalog number EN0321
Price (USD)/ 10,000 units
64.65
Online exclusive
76.75 
Save 12.10 (16%)
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Add to cart
Request bulk or custom format
Price (USD)/ 10,000 units
64.65
Online exclusive
76.75 
Save 12.10 (16%)
Add to cart
S1 Nuclease (100 U/μL)
Catalog numberEN0321
Price (USD)/ 10,000 units
64.65
Online exclusive
76.75 
Save 12.10 (16%)
-
Add to cart
Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is five times more active on DNA than on RNA. S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.

The enzyme is a glycoprotein with carbohydrate content of 18%.

Applications

• Removal of single-stranded overhangs of DNA fragments
• S1 transcript mapping
• Cleavage of hairpin loops
• Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III

Note

S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 U/μL
EnzymeNuclease
Compatible BufferStorage Buffer, 5X Reaction Buffer
Quantity10,000 U
Product TypeS1 Nuclease
Unit Size10,000 units