Thermo Scientific™

RevertAid H Minus Reverse Transcriptase (200 U/µL)

Catalog number:  EP0451

Related applications:

PCR & Real-Time PCR

| Cloning

Back to top

Description

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42 to 45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Related Products
RevertAid H Minus Reverse Transcriptase (200 U/µL)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Optimal Reaction Temperature: 42-45° C
Product Line: RevertAid
Reverse Transcriptase: RevertAid H Minus
Ribonuclease H Activity: None
Size (Final Product): 13 kb or less
Includes: 1mL 5X Reaction Buffer

Contents & storage

RevertAid H Minus Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
Store at -20°C.