Reverse transcription is the synthesis of single-stranded DNA (complementary DNA, or cDNA) using single-stranded RNA as a template, mediated by reverse transcriptases (RTs). The cDNA can be used as a template for amplification by PCR or to generate a cDNA library.

Choosing the correct reverse transcriptase for cDNA synthesis is critical to obtaining high yields of quality, full-length cDNA with full gene representation. With thousands of citations, the Invitrogen™ SuperScript™ family of RTs is a first choice for cDNA synthesis. Use the overview of RTs and cDNA synthesis kit products to choose the solution that’s right for you.

Reverse transcription products

Reverse transcriptase enzymes

A wide range of stand-alone enzymes optimized for reliable, high-quality, full-length cDNA synthesis.

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SuperScript™ IV Reverse Transcriptase

First-strand cDNA synthesis kits

Optimized kit format for simple, time-saving setup; choose based on your downstream application.

Real-time PCR

Cloning and/or RT-PCR

NEW SuperScript IV VILO Master Mix

One-Step RT-PCR

See our one-step RT-PCR formulation with superior sensitivity and a streamlined protocol in a single tube.

Shop one-step RT-PCR kits