Reverse transcription is the process of transcribing RNA molecules into complementary DNA. This cDNA synthesis reaction is mediated by enzymes called reverse transcriptases, or RTs. Moloney murine leukemia virus (M-MLV) reverse transcriptase is one of the RTs used in molecular biology workflows. However, novel engineered RTs are often preferred for enhanced efficiency, improved thermostability, and higher cDNA yields.
Reverse transcription reactions involve multiple steps to synthesize cDNA. Reverse transcriptases exhibit several distinct biochemical activities, predominantly RNA-dependent DNA polymerase activity and RNase H activity. Firstly, reverse transcriptase binds to an RNA template in the presence of an annealed primer. Next, the RNA-dependent DNA polymerase activity of the enzyme synthesizes cDNA by incorporating dNTPs from the reaction mix. This generates a cDNA:RNA hybrid and is referred to as first-strand cDNA synthesis. If reverse transcriptase exhibits RNase H activity (as in wild-type M-MLV RT), the RNA of the cDNA:RNA hybrid is cleaved during first-strand synthesis. In engineered enzymes, RNase H activity is often reduced to prevent premature RNA degradation.
Choose from a variety of reverse transcriptase stand-alone enzymes, master mixes, cDNA synthesis kits, primers, and dNTP solutions to suit your application. Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any type of template RNA.
Use the selection tool to find the right products to meet your cDNA synthesis needs.
Invitrogen SuperScript product family is ideal for all cDNA synthesis experiments. SuperScript IV RT offers top-notch performance marked by thermostability, high processivity, and exceptional cDNA yields, and is available in a selection of formats.
First strand cDNA synthesis master mix crafted with VILO (Variable Input, Linear Output) technology is an ideal choice for two-step RT-qPCR applications. This master mix is known for its remarkable sensitivity, exceptional linearity, and short reaction times.
Skip RNA isolation and expedite cDNA synthesis. Direct reverse transcription products allow cell lysis and cDNA synthesis to be performed in a single tube, using as few as single cell samples.
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