Reverse Transcription
We offer a selection of high-quality products for first-strand cDNA synthesis to meet your research needs. Choose from a variety of reverse transcription enzymes, formats, primers, and dNTP solutions that work best for your application. With our premium line of Invitrogen SuperScript Reverse Transcriptases, we have what you need for superior cDNA synthesis results with any type of template RNA.
Reverse transcription products
Stand-alone enzymes for maximum flexibility in reaction setup
| Superior enzyme for all RNAs, including difficult templates | Value enzyme for commonly used RNA templates | |
| Superscript IV Reverse Transcriptase | M-MLV-Reverse Transcriptase | |
| Optimal reaction temp | 50–55°C | 37°C |
| Reaction time | 10 min | 50 min |
| cDNA yield | Superior | Average |
| Sensitivity | Superior | Average |
| Max cDNA length | Up to 12 kb | Up to 7 kb |
| Ability to work with degraded or inhibitor-containing RNA | High | Low |
| Reduced RNase H activity | Yes | No |
| Learn more | Learn more |
Other reverse transcriptases
Complete kits with all cDNA synthesis reaction components
| Superior choice for all RNAs, including difficult templates | Value choice for commonly used RNA templates | |
| SuperScript IV First-Strand Synthesis System |
High-Capacity cDNA Reverse Transcription Kit | |
| Reverse transcriptase | SuperScript IV | MultiScribe MuLV |
| Optimal reaction temp | 50–55°C | 37°C |
| Reaction time | 10 min | 50 min |
| cDNA yield | Superior | Average |
| Sensitivity | Superior | Average |
| Max cDNA length | Up to 12 kb | Up to 7 kb |
| Ability to work with degraded or inhibitor-containing RNA | High | Low |
| Reduced RNase H activity | Yes | No |
| Learn more | Learn more |
Other cDNA synthesis kits for endpoint PCR applications
cDNA synthesis kits for the highest convenience in RT-qPCR setup
| Superior master mix for all RNA templates | Superior master mix for all RNA templates, with fast and easy gDNA removal | Value choice for commonly used RNA templates | |
| SuperScript IV VILO Master Mix | SuperScript IV VILO Master Mix with ezDNase Enzyme | High-Capacity cDNA Reverse Transcription Kit | |
| Reverse transcriptase | SuperScript IV | SuperScript IV | MultiScribe MuLV |
| Optimal reaction temp | 50°C | 50°C | 37°C |
| Reaction time | 10 min | 10 min | 120 min |
| Ability to work with degraded or inhibitor-containing RNA | High | High | Low |
| Reduced RNase H activity | Yes | Yes | No |
| Primers included | Random primers & oligo(dT)20 | Random primers & oligo(dT)20 | Random primers |
| Integrated gDNA removal | No | Yes | No |
| Format | All RT reaction components premixed | All RT reaction components premixed | RT reaction components in separate tubes |
| Learn more | Learn more | Learn more |
Other cDNA synthesis products for RT-qPCR
One-step RT-PCR kits for fast and simple workflow
| Superior choice for all RNAs, including difficult templates | Value choice for standard applications | |
| SuperScript IV One-Step RT-PCR System | SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase | |
| Reverse transcriptase | SuperScript IV | SuperScript III |
| PCR enzyme | Platinum SuperFi DNA DNA Polymerase | Platinum Taq DNA Polymerase |
| Optimal reaction temperature | 50-55°C | 55°C |
| Reaction time | 10 min | 30 min |
| Sensitivity | Superior | Average |
| Maximum target length | 13.8 kb | 4.5 kb |
| Learn more | Learn more |
Stand-alone enzymes for maximum flexibility in reaction setup
| Superior enzyme for all RNAs, including difficult templates | Value enzyme for commonly used RNA templates | |
| Superscript IV Reverse Transcriptase | M-MLV-Reverse Transcriptase | |
| Optimal reaction temp | 50–55°C | 37°C |
| Reaction time | 10 min | 50 min |
| cDNA yield | Superior | Average |
| Sensitivity | Superior | Average |
| Max cDNA length | Up to 12 kb | Up to 7 kb |
| Ability to work with degraded or inhibitor-containing RNA | High | Low |
| Reduced RNase H activity | Yes | No |
| Learn more | Learn more |
Other reverse transcriptases
Complete kits with all cDNA synthesis reaction components
| Superior choice for all RNAs, including difficult templates | Value choice for commonly used RNA templates | |
| SuperScript IV First-Strand Synthesis System |
High-Capacity cDNA Reverse Transcription Kit | |
| Reverse transcriptase | SuperScript IV | MultiScribe MuLV |
| Optimal reaction temp | 50–55°C | 37°C |
| Reaction time | 10 min | 50 min |
| cDNA yield | Superior | Average |
| Sensitivity | Superior | Average |
| Max cDNA length | Up to 12 kb | Up to 7 kb |
| Ability to work with degraded or inhibitor-containing RNA | High | Low |
| Reduced RNase H activity | Yes | No |
| Learn more | Learn more |
Other cDNA synthesis kits for endpoint PCR applications
cDNA synthesis kits for the highest convenience in RT-qPCR setup
| Superior master mix for all RNA templates | Superior master mix for all RNA templates, with fast and easy gDNA removal | Value choice for commonly used RNA templates | |
| SuperScript IV VILO Master Mix | SuperScript IV VILO Master Mix with ezDNase Enzyme | High-Capacity cDNA Reverse Transcription Kit | |
| Reverse transcriptase | SuperScript IV | SuperScript IV | MultiScribe MuLV |
| Optimal reaction temp | 50°C | 50°C | 37°C |
| Reaction time | 10 min | 10 min | 120 min |
| Ability to work with degraded or inhibitor-containing RNA | High | High | Low |
| Reduced RNase H activity | Yes | Yes | No |
| Primers included | Random primers & oligo(dT)20 | Random primers & oligo(dT)20 | Random primers |
| Integrated gDNA removal | No | Yes | No |
| Format | All RT reaction components premixed | All RT reaction components premixed | RT reaction components in separate tubes |
| Learn more | Learn more | Learn more |
Other cDNA synthesis products for RT-qPCR
One-step RT-PCR kits for fast and simple workflow
| Superior choice for all RNAs, including difficult templates | Value choice for standard applications | |
| SuperScript IV One-Step RT-PCR System | SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase | |
| Reverse transcriptase | SuperScript IV | SuperScript III |
| PCR enzyme | Platinum SuperFi DNA DNA Polymerase | Platinum Taq DNA Polymerase |
| Optimal reaction temperature | 50-55°C | 55°C |
| Reaction time | 10 min | 30 min |
| Sensitivity | Superior | Average |
| Maximum target length | 13.8 kb | 4.5 kb |
| Learn more | Learn more |
Featured videos
Resources
Education
Review educational resources for reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications.
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View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR, and cloning.
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OEM & customized cDNA solutions
Tailor-made performance tested products with a strict quality standards to meet your assay needs.
Support
PCR and cDNA Synthesis Support Center
Find tips, troubleshooting help, and resources for your endpoint PCR and cDNA applications.
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For Research Use Only. Not for use in diagnostic procedures.