Reverse Transcription

We offer a selection of high-quality products for first-strand cDNA synthesis to meet your research needs. Choose from a variety of reverse transcription enzymes, formats, primers, and dNTP solutions that work best for your application. With our premium line of Invitrogen SuperScript Reverse Transcriptases, we have what you need for superior cDNA synthesis results with any type of template RNA.

Reverse transcription products

Stand-alone enzymes for maximum flexibility in reaction setup

	Superior enzyme for all RNAs, including difficult templates	Value enzyme for commonly used RNA templates
	Superscript IV Reverse Transcriptase	M-MLV-Reverse Transcriptase
Optimal reaction temp	50–55°C	37°C
Reaction time	10 min	50 min
cDNA yield	Superior	Average
Sensitivity	Superior	Average
Max cDNA length	Up to 12 kb	Up to 7 kb
Ability to work with degraded or inhibitor-containing RNA	High	Low
Reduced RNase H activity	Yes	No
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Other reverse transcriptases

Complete kits with all cDNA synthesis reaction components

	Superior choice for all RNAs, including difficult templates	Value choice for commonly used RNA templates
	SuperScript IV First-Strand Synthesis System	High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptase	SuperScript IV	MultiScribe MuLV
Optimal reaction temp	50–55°C	37°C
Reaction time	10 min	50 min
cDNA yield	Superior	Average
Sensitivity	Superior	Average
Max cDNA length	Up to 12 kb	Up to 7 kb
Ability to work with degraded or inhibitor-containing RNA	High	Low
Reduced RNase H activity	Yes	No
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Other cDNA synthesis kits for endpoint PCR applications

SuperScript III First-Strand cDNA System for RT-PCR

cDNA synthesis kits for the highest convenience in RT-qPCR setup

	Superior master mix for all RNA templates	Superior master mix for all RNA templates, with fast and easy gDNA removal	Value choice for commonly used RNA templates
	SuperScript IV VILO Master Mix	SuperScript IV VILO Master Mix with ezDNase Enzyme	High-Capacity cDNA Reverse Transcription Kit
Reverse transcriptase	SuperScript IV	SuperScript IV	MultiScribe MuLV
Optimal reaction temp	50°C	50°C	37°C
Reaction time	10 min	10 min	120 min
Ability to work with degraded or inhibitor-containing RNA	High	High	Low
Reduced RNase H activity	Yes	Yes	No
Primers included	Random primers & oligo(dT)₂₀	Random primers & oligo(dT)₂₀	Random primers
Integrated gDNA removal	No	Yes	No
Format	All RT reaction components premixed	All RT reaction components premixed	RT reaction components in separate tubes
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Other cDNA synthesis products for RT-qPCR

One-step RT-PCR kits for fast and simple workflow

	High-performing choice for high-fidelity applications	High-performing choice for standard applications
	SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity	SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Reverse transcriptase	SuperScript III	SuperScript III
PCR enzyme	Platinum Taq High Fidelity DNA Polymerase	Platinum Taq DNA Polymerase
Optimal reaction temp	55°C	55°C
Reaction time	30 min	30 min
Sensitivity	High	High
Maximum target length	Up to 10 kb	Up to 4.5 kb
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