Invitrogen™

M-MLV Reverse Transcriptase (200 U/µL)

Catalog number: 28025013
Invitrogen™

M-MLV Reverse Transcriptase (200 U/µL)

Catalog number: 28025013
Catalog Number
28025013
Unit Size
40,000 units
Price (USD)
Price: 334.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200,000 units
Price (USD)
Price: 1,508.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2802501340,000 units
Price: 334.00
Your Price:
***
28025021200,000 units
Price: 1,508.00
Your Price:
***
Product Overview
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Additional Information
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M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity. Features of this enzyme:

Thermostability—optimal activity at 37°C
Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7 kb
Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR

Source
Purified from E. coli expressing the pol gene of M-MLV on a plasmid

Performance and quality testing
SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product

Unit definition
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A)oligo(dT)25 as template-primer.

Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 µL for 10 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
200 U/μL
Final Product Type
cDNA (First-Strand)
Format
Stand-alone enzyme
GC-Rich PCR Performance
Low
No. of Reactions
200 Reactions
PCR Method
RT-PCR, RT-qPCR
Reaction Format
Separate components
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
7 kb or Less
Starting Material
RNA
Technique
Reverse Transcription
Volume
200 μL
Enzyme Function
RNA dependent DNA Polymerase
Includes
1 x 200μL M-MLV RT (200U/μL); 1mL 5X First Strand Buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM Magnesium Chloride]; 500μL 100mM DTT; 40,000 units
No. of Tests
200 Reactions
Optimal Reaction Temperature
37°C
Product Type
M-MLV Reverse Transcriptase
Quantity
200 Reactions; 40,000 units
Reverse Transcriptase
M-MLV
Ribonuclease H Activity
Reduced
Sensitivity
Low
Shipping Condition
Wet Ice
Sufficient For
200 Reactions
Template
ssDNA, ssRNA, RNA-DNA Hybrid

Contents & Storage

Contains:
• 1 x 200 μl M-MLV RT (200 U/μl)
• 1 ml 5X First Strand Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM Magnesium Chloride]
• 500 μl 100 mM DTT

Store at -20°C.