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Reverse Transcription Products
Shop from our array of reverse transcription products designed for efficient RNA-to-cDNA conversion in molecular biology workflows. This category includes cDNA synthesis kits, master mixes, reverse transcriptases, and one-step RT-PCR reagents. These products are essential for various applications, including gene expression analysis, cloning, and pathogen detection. Selection of reverse transcriptases include wild type MMLV-derived enzymes and engineered MMLV RT variants with high thermal stability, reduced RNase H activity and enhanced processivity for improved cDNA yields. cDNA synthesis kits and master mixes provide all components necessary for first-strand cDNA synthesis in a convenient and optimized format. One-step RT-PCR reagents combine reverse transcription and PCR amplification in a single reaction, streamlining workflows for RNA detection and quantification. Learn more about reverse transcription.
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cDNA Synthesis Kits and Master Mixes (26)
Reverse Transcriptases (10)
One-Step Reverse Transcription PCR Reagents (6)
Products (42)
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The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Concentration: 200 U/μL. Final Product Type: First-Strand cDNA. Format: Stand-alone Enzyme. Optimal Reaction Temperature: 50°C.
Final Product Type: First-Strand cDNA. Format: Kit. No. of Reactions: 50 Reactions. Optimal Reaction Temperature: 50°C.
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The system includes SuperScript IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and...
Final Product Type: First-Strand cDNA. Format: Kit. Optimal Reaction Temperature: 42°C. Reaction Format: Master Mix.
Concentration: 50 U/μL. Final Product Type: First-Strand cDNA. Format: Kit. Optimal Reaction Temperature: 42°C.
Format: Stand-alone Enzyme. GC-Rich PCR Performance: High. Reaction Speed: 50 min. Technique: Reverse Transcription.
Final Product Type: First-Strand cDNA. Format: Kit. Optimal Reaction Temperature: 42°C. Reaction Format: Separate components.
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide...
Concentration: 5X. Final Product Type: First-Strand cDNA. Format: Master Mix. Optimal Reaction Temperature: 50°C.
Final Product Type: PCR Amplified cDNA. Format: Kit. Optimal Reaction Temperature: 50°C. Polymerase: Platinum SuperFi U DNA Polymerase.
Format: Stand-alone Enzyme. GC-Rich PCR Performance: High. Reaction Speed: 30 to 50 min. Technique: Reverse Transcription.
Concentration: 4X. Final Product Type: First-Strand cDNA. Format: Master Mix. Reagent Type: Reverse Transcription.
Concentration: 200 U/μL. Final Product Type: First-Strand cDNA. Format: Stand-alone Enzyme. Optimal Reaction Temperature: 42°C.
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Reverse transcription is the process of transcribing RNA molecules into complementary DNA. This cDNA synthesis reaction is mediated by enzymes called reverse transcriptases, or RTs. Moloney murine leukemia virus (M-MLV) reverse transcriptase is one of the RTs used in molecular biology workflows.
Once RNA is quantified, you are ready for reverse transcription. The ideal reverse transcriptase (RT) will reverse-transcribe even the most difficult RNAs, such as those degraded during the purification process or those in limited quantity.
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What is the longest cDNA product that Superscript IV RT can synthesize?
Product Sheet: TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control