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Invitrogen
TRA-1-81 Monoclonal Antibody (TRA-1-81), HRP
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FIGURE: 1 / 1
TRA-1-81 Antibody (MA1-024-HRP) in WB
Western blot analysis of TRA-1-81 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
Product Details
MA1-024-HRP
Product Specifications
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TRA-1-81
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-024-HRP has been successfully used in Western blot applications on human samples.
Target Information
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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