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ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor™ 647
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FIGURE: 1 / 7
ATP1A1 Antibody (MA3-928-A647) in ICC/IF
Immunofluorescent analysis of Na/K ATPase Alpha (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-928-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 ... View More
Product Details
MA3-928-A647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Sheep,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M7-PB-E9
Immunogen
Purified alpha sodium/potassium ATPase from sheep kidney
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3928A647 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.
The MA3928A647 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.
Target Information
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; G00-119-711; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+ K+ ATPase alpha subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product
Gene Aliases: ATP1A1; Atpa-1; BC010319; CMT2DD; HOMGSMR2; Nkaa1b
UniProt ID: (Sheep) P04074, (Human) P05023, (Pig) P05024, (Bovine) Q08DA1, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2
Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Human) 476, (Pig) 397481, (Bovine) 282144, (Chicken) 396530, (Rat) 24211, (Mouse) 11928
sodium:potassium-exchanging ATPase activity
ATP binding
metal ion binding
nucleotide binding
protein binding
potassium-transporting ATPase activity
phosphatase activity
ATPase activity
potassium ion binding
sodium ion binding
ion channel binding
protein heterodimerization activity
chaperone binding
steroid hormone binding
ATPase binding
protein kinase binding
protein domain specific binding
ankyrin binding
ADP binding
phosphatidylinositol 3-kinase binding
hydrolase activity
primary active transporter
regulation of sodium ion transport
sodium ion transport
establishment or maintenance of transmembrane electrochemical gradient
potassium ion transmembrane transport
regulation of the force of heart contraction
ion transport
potassium ion transport
cellular sodium ion homeostasis
osmosensory signaling pathway
regulation of blood pressure
response to xenobiotic stimulus
cellular cation homeostasis
cellular potassium ion homeostasis
negative regulation of glucocorticoid biosynthetic process
sodium ion transmembrane transport
sodium ion export from cell
negative regulation of heart contraction
positive regulation of heart contraction
positive regulation of striated muscle contraction
sodium ion homeostasis
relaxation of cardiac muscle
heart contraction
cellular response to steroid hormone stimulus
cardiac muscle cell action potential involved in contraction
membrane repolarization
membrane repolarization during cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
hydrogen ion transmembrane transport
response to glycoside
potassium ion import across plasma membrane
transmembrane transport
potassium ion import
energy coupled proton transmembrane transport, against electrochemical gradient
dephosphorylation
response to drug
cardiac muscle contraction
membrane hyperpolarization
cellular response to mechanical stimulus
regulation of cardiac muscle cell contraction
transport
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