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Invitrogen

Bcl-2 Monoclonal Antibody (100/D5)

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53 Published Figures
91 References
View all (169) BCL-2 antibodies

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Bcl-2 Monoclonal Antibody (100/D5)

Product Details

MA5-11757

Applications
Tested Dilution
Publications

Western Blot (WB)

1:50-1:200
View 43 publications 43 publications

Immunohistochemistry (IHC)

-
View 33 publications 33 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:10-1:100
View 8 publications 8 publications

Flow Cytometry (Flow)

0.5-1 µg/test
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

100/D5

Immunogen

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978135

Product Specific Information

MA5-11757 targets BCL-2 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.

The MA5-11757 immunogen is a synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

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UniProt ID: (Human) P10415, (Mouse) P10417

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Entrez Gene ID: (Human) 596, (Mouse) 12043

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Function(s)
protease binding protein binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding binding, bridging DNA-binding transcription factor binding transcription factor binding protein homodimerization activity BH domain binding
Process(es)
G1/S transition of mitotic cell cycle protein polyubiquitination mitotic cell cycle cell morphogenesis ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response leukocyte homeostasis B cell homeostasis B cell apoptotic process kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development negative regulation of B cell apoptotic process response to ischemia renal system process melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis autophagy apoptotic process humoral immune response cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis female pregnancy cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to radiation response to xenobiotic stimulus response to toxic substance post-embryonic development organ morphogenesis response to iron ion response to UV-B response to gamma radiation regulation of gene expression regulation of autophagy negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation smooth muscle cell migration positive regulation of smooth muscle cell migration cell migration cochlear nucleus development gland morphogenesis hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction regulation of protein localization myeloid cell apoptotic process negative regulation of myeloid cell apoptotic process T cell differentiation in thymus osteoblast proliferation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth regulation of growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to hydrogen peroxide neuron maturation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process regulation of programmed cell death CD8-positive, alpha-beta T cell lineage commitment pigmentation negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle retinal cell programmed cell death negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development skeletal muscle fiber development positive regulation of skeletal muscle fiber development pigment granule organization stem cell development homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus establishment of localization in cell regulation of cell cycle regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport hematopoietic stem cell differentiation calcium ion transport into cytosol intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress lymphocyte apoptotic process negative regulation of lymphocyte apoptotic process T cell apoptotic process negative regulation of T cell apoptotic process cellular response to hypoxia reactive oxygen species metabolic process dendritic cell apoptotic process motor neuron apoptotic process extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of intracellular signal transduction epithelial cell apoptotic process negative regulation of epithelial cell apoptotic process negative regulation of G1/S transition of mitotic cell cycle negative regulation of dendritic cell apoptotic process negative regulation of motor neuron apoptotic process negative regulation of anoikis regulation of apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway response to acid chemical protein dephosphorylation cell aging cell growth single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of transmembrane transporter activity positive regulation of peptidyl-serine phosphorylation growth response to drug positive regulation of catalytic activity regulation of protein homodimerization activity regulation of protein heterodimerization activity regulation of catalytic activity transmembrane transport cellular response to organic substance negative regulation of reactive oxygen species metabolic process
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