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Invitrogen
MAP2 Monoclonal Antibody (AP20)
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FIGURE: 1 / 9
MAP2 Antibody (MA5-12823) in ICC/IF
Immunofluorescence analysis of neurons using anti-MAP2 antibody (Product # MA5-12823). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against MAP2 (Product # MA5-12823 at 1:200) followed by Alexa Fluor 488 goat anti-rabbit (Product # A-21206, green). Nuclear DNA was stained with DAPI (blue) in the merged image (right panel).
Product Details
MA5-12823
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AP20
Immunogen
Bovine brain microtubule associated protein 2
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c). The epitope has been mapped to aa 997-1332.
The molecular weight of the doublet is ~280 kDa.
For IHC staining, formalin-fixed tissues must be boiled in a 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommened positive controls include human glioblastoma T98G cells or brain tissue.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; microtubule associated protein 2; microtubule associated protein 2C; Microtubule-associated protein 2; microtubule-associated protein-2; OTTHUMP00000163916; OTTHUMP00000208143; unnamed protein product
Gene Aliases: MAP-2; MAP2; MAP2A; MAP2B; MAP2C
UniProt ID: (Human) P11137
Entrez Gene ID: (Bovine) 281294, (Human) 4133
microtubule cytoskeleton organization
dendrite development
central nervous system neuron development
negative regulation of axon extension
regulation of microtubule polymerization
negative regulation of microtubule polymerization
neuron projection development
regulation of protein localization
dendrite morphogenesis
positive regulation of anterograde dense core granule transport
regulation of organelle transport along microtubule
positive regulation of anterograde synaptic vesicle transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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