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Blimp-1 Monoclonal Antibody (3H2-E8), DyLight™ 550

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Blimp-1 Monoclonal Antibody (3H2-E8), DyLight™ 550

Product Details

MA5-16117

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

8 µg/mL
-

ELISA (ELISA)

1:100-1:2,000
-

Immunoprecipitation (IP)

Assay-dependent
-

ChIP assay (ChIP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3H2-E8

Immunogen

A fragment of mouse Blimp-1 corresponding to residues 199-409.
3D Epitope / Immunogen

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

Excitation/Emission Max

556/570 nm View spectra spectra

Form

Liquid

Concentration

0.80 mg/mL

Amount

80 µg

Purification

Protein G

Storage buffer

50mM sodium borate

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152755

Product Specific Information

In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).

Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.

This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.

Suggested positive control: P3X (murine plasmocytoma) cell lysates.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1 with ZNF domain isoform 2; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-BF1 alpha; PRDI-BF1 beta; PRDI-binding factor-1; RP1-134E15.1; unnamed protein product

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Gene Aliases: b2b1765Clo; BLIMP-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1

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UniProt ID: (Human) O75626, (Mouse) Q60636

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Entrez Gene ID: (Pig) 100154284, (Human) 639, (Mouse) 12142

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding methyltransferase activity zinc ion binding transferase activity metal ion binding histone methyltransferase binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding C2H2 zinc finger transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development morphogenesis of a branching structure kidney development embryonic placenta development maternal placenta development adaptive immune response immune system process heart valve development cardiac septum development ventricular septum development regulation of transcription from RNA polymerase II promoter germ cell development post-embryonic development gene expression positive regulation of gene expression negative regulation of gene expression methylation regulation of natural killer cell differentiation regulation of extrathymic T cell differentiation aorta development regulation of cell proliferation eye photoreceptor cell development innate immune response cell fate commitment artery morphogenesis cellular developmental process regulation of NK T cell differentiation retinal bipolar neuron differentiation intestinal epithelial cell development trophoblast giant cell differentiation coronary vasculature development sebum secreting cell proliferation
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