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Invitrogen
NLK Recombinant Rabbit Monoclonal Antibody (24GB585)
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FIGURE: 1 / 4
NLK Antibody (MA5-57503) in ICC/IF
Immunocytochemical staining of NLK in HeLa cells using NLK Recombinant Monoclonal Antibody (Product # MA5-57502) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Nemo like kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57503
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB585
Immunogen
A synthesized peptide derived from human NLK (aa 430-495)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NLK; similar to mouse nemo-like kinase (Nlk); unnamed protein product
UniProt ID: (Human) Q9UBE8
Entrez Gene ID: (Human) 51701
MAPK cascade
regulation of transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
regulation of signal transduction
peptidyl-threonine phosphorylation
negative regulation of Wnt signaling pathway
cellular response to stress
intracellular signal transduction
protein stabilization
cellular response to osmotic stress
negative regulation of TORC1 signaling
positive regulation of STAT cascade
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