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AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
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| Catalog Number | Includes | Quantity |
|---|---|---|
| N8080161 | Buffer II | 250 Units |
| N8080172 | Buffer II | 1000 Units |
| N8080153 | Buffer II | 3,000 units |
| N8080156 | Buffer II | 5,000 units |
| N8080186 | Buffer II | 25,000 units |
| N8080152 | Buffer I | 3,000 units |
| N8080155 | Buffer I | 5,000 units |
| N8080160 | Buffer I | 250 Units |
| N8080171 | Buffer I | 1,000 units |
| N8080185 | Buffer I | 25,000 Units |
Catalog number N8080161
Price (USD)/ Each
141.65
Online Exclusive
150.00Save 8.35 (6%)
Includes:
Buffer II
Quantity:
250 Units
Price (USD)/ Each
141.65
Online Exclusive
150.00Save 8.35 (6%)
AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.
AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl2 Solution.
Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.
Features
- The most thoroughly characterized enzyme available for the PCR process
- Excellent thermal activity profile makes it good for PCR applications
- QC-tested to guarantee reproducible results
Notes
- For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone enzyme
PolymeraseAmpliTaq DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Product TypeDNA Polymerase
Quantity250 Units
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
IncludesBuffer II
Unit SizeEach
Contents & Storage
• AmpliTaq DNA Polymerase (5 U/μL), 50 μL
• GeneAmp 10X PCR Buffer II, 1.5 mL
• 25 mM MgCl2, 1.5 mL
Store at -20°C.
• GeneAmp 10X PCR Buffer II, 1.5 mL
• 25 mM MgCl2, 1.5 mL
Store at -20°C.