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Applied Biosystems™
AmpliTaq™ DNA Polymerase with Buffer II (250 units/tube)
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Description
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.Features of this enzyme:
Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II. It is also available with GeneAmp 10X PCR Buffer I and MgCl2 Solution.
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Polymerase: | AmpliTaq® DNA Polymerase |
|---|---|
| Hot Start: | No |
| Fidelity (vs. Taq): | 1 X |
| Reaction Format: | Separate Components |
| Product Overhang: | 3'-A |
| Reaction Speed: | Standard |
| Final Product Size(s): | 5 kb or less |
| Exonuclease Activity: | 5' - 3' |
| Product Line: | AmpliTaq™ |
| Product Size: | 250 units |
| Shipping Condition: | Dry Ice |
Contents & storage
Contents:
- 50 µL of AmpliTaq® DNA Polymerase (5 U/µL)
- 1.5 mL of GeneAmp® 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
- 1.5 mL 25 mM Magnesium Chloride
