Applied Biosystems™

      AmpliTaq™ DNA Polymerase with Buffer II (250 units/tube)

      AmpliTaq™ DNA Polymerase with Buffer II (250 units/tube)

      Catalog number:  N8080161

       Related applications: PCR

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      Description

      AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

      Features of this enzyme:

    • AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
    • Its thermal activity profile makes it good for PCR applications
    • It is QC-tested to guarantee reproducible results

      • Reliable and robust PCR
      The thermal activity profile of AmpliTaq® DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Polymerase: AmpliTaq® DNA Polymerase
      Hot Start: No
      Fidelity (vs. Taq): 1 X
      Reaction Format: Separate Components
      Product Overhang: 3'-A
      Reaction Speed: Standard
      Final Product Size(s): 5 kb or less
      Exonuclease Activity: 5' - 3'
      Product Line: AmpliTaq™
      Product Size: 250 units
      Shipping Condition: Dry Ice

      Contents & storage

      Contents:
      • 50 µL of AmpliTaq® DNA Polymerase (5 U/µL)
      • 1.5 mL of GeneAmp® 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
      • 1.5 mL 25 mM Magnesium Chloride
      Store at -20°C.

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