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Applied Biosystems™
AmpliTaq™ DNA Polymerase with Buffer II
Description
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.Features of this enzyme:
Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Exonuclease Activity: | 5' - 3' |
|---|---|
| Hot Start: | No |
| Product Line: | AmpliTaq™ |
| Quantity: | 250 units |
| Shipping Condition: | Dry Ice |
| Concentration: | 5 U/µL, 10X |
| Detection Method: | Primer-Probe |
| Fidelity (vs. Taq): | 1 X |
| For Use With (Application): | PCR Amplification |
| Overhang: | 3'-A |
| Polymerase: | AmpliTaq™ DNA Polymerase |
| Product Type Specs: | DNA Polymerase |
| Reaction Format: | Separate Components |
| Reaction Speed: | Standard |
| Size (Final Product): | 5 kb or less |
| Volume: | 1.5 L |
Contents & storage
Contents:
- 50 µL of AmpliTaq™ DNA Polymerase (5 U/µL)
- 1.5 mL of GeneAmp™ 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
- 1.5 mL 25 mM Magnesium Chloride
