Switch to Thermo Scientific DreamTaq DNA Polymerases for better results and added convenience.
Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer II (250 units/tube)

Catalog number:  N8080161

 Related applications:


Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II. It is also available with GeneAmp 10X PCR Buffer I and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    For Research Use Only. Not for use in diagnostic procedures.


    Polymerase: AmpliTaq® DNA Polymerase
    Hot Start: No
    Fidelity (vs. Taq): 1 X
    Reaction Format: Separate Components
    Product Overhang: 3'-A
    Reaction Speed: Standard
    Final Product Size(s): 5 kb or less
    Exonuclease Activity: 5' - 3'
    Product Line: AmpliTaq™
    Product Size: 250 units
    Shipping Condition: Dry Ice

    Contents & storage

    • 50 µL of AmpliTaq® DNA Polymerase (5 U/µL)
    • 1.5 mL of GeneAmp® 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
    • 1.5 mL 25 mM Magnesium Chloride
    Store at -20°C.


    Manuals & protocols