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Invitrogen
MAP2 Polyclonal Antibody
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FIGURE: 1 / 10
MAP2 Antibody (PA5-17646) in ICC/IF
Immunofluorescence analysis of MAP2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Polyclonal Antibody (Product # PA5-17646) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
Product Details
PA5-17646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to carboxy-terminal residues of human MAP2
Conjugate
Form
Liquid
Concentration
73.6 µg/mL
Amount
7.36 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
PA5-17646 was successfully used to detect MAP2 in neurons differentiated from H9 ESC derived NSCs.
This antibody is not cross-reactive with tau.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; microtubule associated protein 2; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143; unnamed protein product
Gene Aliases: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4
UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146
Entrez Gene ID: (Human) 4133, (Mouse) 17756, (Rat) 25595
microtubule bundle formation
axonogenesis
dendrite development
cellular response to organic substance
central nervous system neuron development
neuron projection development
response to estradiol
response to progesterone
dendrite morphogenesis
microtubule cytoskeleton organization
negative regulation of axon extension
regulation of microtubule polymerization
negative regulation of microtubule polymerization
regulation of protein localization
positive regulation of anterograde dense core granule transport
regulation of organelle transport along microtubule
positive regulation of anterograde synaptic vesicle transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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