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Invitrogen
HGS Polyclonal Antibody
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FIGURE: 1 / 3
HGS Antibody (PA5-104477) in ICC/IF
Immunofluorescent analysis of HGS in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and HGS polyclonal antibody (Product # PA5-104477) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104477
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HGS(Accession O14964), corresponding to amino acid residues S310-Q360.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HGS.
Target Information
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGF-regulated tyrosine kinase substrate; HGNC:4897; human growth factor-regulated tyrosine kinase substrate; protein pp110; SNAP-25-interacting protein Hrs-2; unnamed protein product
Gene Aliases: Hgr; HRS; tn; ZFYVE8
UniProt ID: (Human) O14964, (Mouse) Q99LI8, (Rat) Q9JJ50
Entrez Gene ID: (Human) 9146, (Mouse) 15239, (Rat) 56084
protein targeting to lysosome
signal transduction
negative regulation of cell proliferation
membrane invagination
positive regulation of gene expression
negative regulation of platelet-derived growth factor receptor signaling pathway
endosomal transport
macroautophagy
negative regulation of angiogenesis
negative regulation of vascular endothelial growth factor receptor signaling pathway
receptor internalization
endocytic recycling
multivesicular body assembly
regulation of protein catabolic process
protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of MAP kinase activity
negative regulation of JAK-STAT cascade
protein localization to membrane
membrane fission
positive regulation of exosomal secretion
endosome to lysosome transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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