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VWF Polyclonal Antibody
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FIGURE: 1 / 3
VWF Antibody (PA5-104687) in ICC/IF
Immunocytochemistry analysis of VWF in HepG2 cells (30 min of 4 µM Forskolin treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with VWF polyclonal antibody (Product # PA5-104687) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104687
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human VWF(Accession P04275), corresponding to amino acid residues N1005-M1055.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total VWF.
Target Information
Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adhesive protein; adhesive protein; VWF; Coagulation Factor VIII; coagulation factor VIII VWF; Factor VIII related antigen; unnamed protein product; von Willebrand antigen II precursor; von Willebrand Disease (vWD); von Willebrand factor; Von Willebrand factor homolog
Gene Aliases: 6820430P06Rik; B130011O06Rik; C630030D09; F8VWF; VWD
UniProt ID: (Human) P04275, (Mouse) Q8CIZ8
Entrez Gene ID: (Human) 7450, (Rat) 116669, (Mouse) 22371
liver development
placenta development
activation of blood coagulation via clotting cascade
inflammatory response
cell adhesion
blood coagulation
hemostasis
response to radiation
response to X-ray
platelet activation
extracellular matrix organization
cell-substrate adhesion
response to L-ascorbic acid
response to immobilization stress
cellular response to cold
cellular response to lipopolysaccharide
liver regeneration
positive regulation of platelet aggregation
positive regulation of intracellular signal transduction
response to wounding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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