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Invitrogen
E-cadherin Polyclonal Antibody
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FIGURE: 1 / 21
E-cadherin Antibody (PA5-32178) in ICC/IF
Immunofluorescence analysis of E-cadherin was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with E-cadherin Polyclonal Antibody (Product # PA5-32178) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More
Product Details
PA5-32178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Protozoa,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human E-Cadherin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Amount
109 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, PC-12, MCF-7.
Predicted reactivity: Rat (80%), Pig (82%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-cadherin; E-cadherin 1; Ecadherin; epididymis secretory sperm binding protein; Epithelial cadherin; unnamed protein product; Uvomorulin
Gene Aliases: Arc-1; BCDS1; CD324; CDH1; CDHE; ECAD; LCAM; UVO
UniProt ID: (Human) P12830, (Rat) Q9R0T4
Entrez Gene ID: (Human) 999, (Rat) 83502
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
identical protein binding
gamma-catenin binding
cadherin binding
metal ion binding
cell adhesion molecule binding
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
glycoprotein binding
protein phosphatase binding
protein domain specific binding
cadherin
cell morphogenesis
desmosome assembly
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to xenobiotic stimulus
response to toxic substance
regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
pituitary gland development
negative regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of axon extension
neuron projection development
cell junction assembly
adherens junction organization
positive regulation of protein import into nucleus
cell-cell adhesion mediated by cadherin
positive regulation of transcription, DNA-templated
regulation of transport
cellular response to lithium ion
response to heparin
cellular response to indole-3-methanol
protein localization to plasma membrane
cell-cell adhesion
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
response to Gram-positive bacterium
positive regulation of cellular protein localization
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
sensory perception of sound
response to organic substance
single organismal cell-cell adhesion
protein metabolic process
regulation of protein localization
regulation of water loss via skin
response to drug
positive regulation of transcription factor import into nucleus
regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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