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Invitrogen
Armenian Hamster IgG Isotype Control, PE
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Product Details
PA5-33222
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Armenian hamster
/ IgG
Class
Control
Type
Isotype Control
Immunogen
Trinitrophenol - KLH
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
PA5-33222 is suitable for use as a negative control for the measurement of non-specific binding of Armenian hamster monoclonal antibodies, of isotype IgG, in human, mouse or rat tissues.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
In Flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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