Invitrogen™

ProQuest™ Two-Hybrid System with Gateway™ Technology

Catalog number:  PQ1000101

Related applications:

Protein Assays and Analysis

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Description

The ProQuest™ Two-Hybrid System with Gateway® Technology is an in vivo yeast-based system for identifying interactions between two proteins. This interaction reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media.
The ProQuest™ System features:
• Gateway® Technology to allow rapid and easy generation of bait and prey constructs, and to facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (HIS3, URA3, and lacZ) with independent promoter regions to rapidly weed out false positives. URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid.

• A set of strong-to-weak Gateway®-based control interactions to evaluate results and assess the strength of the interaction being tested
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Two-Hybrid Assay
Expression System: Yeast
Product Line: Gateway®, ProQuest™
System Type: ProQuest™
Reporter Gene: Beta-Gal (lacZ), HIS3, URA3

Contents & storage

The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase® II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored.