Invitrogen™

Qubit™ RNA IQ Assay Kit

Catalog number:  Q33222

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using the Qubit 4 Fluorometer (required). The assay utilizes two unique dyes: one that binds to large, intact and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality, and also as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Solution-based compared to electrophoresis methods
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time to run. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended to obtain fragment size information or size distribution of the RNA sample.

Notes:
1. The Qubit RNA IQ assay will only work on the Qubit 4 Fluorometer. It will not work on the Qubit, Qubit 2.0, or Qubit 3 fluorometers.
2. 500 µL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Volume: 1-20 µL (0.5-1.5 µg)
For Use With (Equipment): Qubit™ 4 Fluorometer
Product Line: Qubit™
Product Size: 275 assays
Sample Type (General): RNA
Shipping Condition: Dry Ice

Contents & storage

Store kit at -20°C or below. Store RNA standards at -80°C or below.

Documents

Manuals & protocols