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Invitrogen
Human Tartrate Resistant Acid Phosphatase (aa 35-121) Control Fragment Recombinant Protein
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Product Details
RP-103307
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GGVPNAPFHTAREMANAKEIARTVQILGADFILSLGDNFYFTGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQI
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
5.2 mg/mL
Amount
520 µg
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84356. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression is increased in spleen and monocytes of patients with Gaucher's disease; spleenocytes and circulating leukocytes of patients with hairy cell leukemia; spleen of patients with Hodgkin's lymphoma and in the sera of patients undergoing active bone turnover.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACP5; human purple acid phosphatase; Tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5a; tartrate-resistant acid phosphatase 5b; Tartrate-resistant acid phosphatase type 5; TR-AP; TRAPase; TrATPase; Type 5 acid phosphatase; unnamed protein product
Gene Aliases: ACP5; HPAP; TRAcP; TRACP5a; TRACP5b; TRAP; TrATPase
UniProt ID: (Human) P13686
Entrez Gene ID: (Human) 54
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