RH 237 (N-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt)

RH 237 (<i>N</i>-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt)

Citations & References (25)

Invitrogen™

RH 237 (N-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt)

The fast-response potential-sensitive probe RH 237 can be used for imaging functional neurons.Read more

Catalog Number	Quantity
S1109	5 mg

Catalog number S1109

Price (MXN)

Quantity:

The fast-response potential-sensitive probe RH 237 can be used for imaging functional neurons.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorInfrared

Detection MethodFluorescence

For Use With (Equipment)Fluorescence Microscope

Product TypeSensitive Probe

Quantity5 mg

Shipping ConditionRoom Temperature

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (25)

Citations & References

Abstract

Imaging of cerebellar surface activation in vivo using voltage sensitive dyes.

Authors:Kim JH, Dunn MB, Hua Y, Rydberg J, Yae H, Elias SA, Ebner TJ

Journal:Neuroscience

PubMed ID:2594193

'The understanding of the information processing performed by complex neuronal networks in the central nervous system will require techniques permitting the simultaneous monitoring of the electrical activity of neuronal ensembles. Voltage sensitive dyes offer the potential for non-invasive optical monitoring of the activity in large populations of neurons. In this ... More

Dynamic origin of spatially discordant alternans in cardiac tissue.

Authors:Hayashi H, Shiferaw Y, Sato D, Nihei M, Lin SF, Chen PS, Garfinkel A, Weiss JN, Qu Z

Journal:Biophys J

PubMed ID:17071663

'Alternans, a condition in which there is a beat-to-beat alternation in the electromechanical response of a periodically stimulated cardiac cell, has been linked to the genesis of life-threatening ventricular arrhythmias. Optical mapping of membrane voltage (V(m)) and intracellular calcium (Ca(i)) on the surface of animal hearts reveals complex spatial patterns ... More

Fluorescence imaging of local membrane electric fields during the excitation of single neurons in culture.

Authors:Gogan P, Schmiedel-Jakob I, Chitti Y, Tyc-Dumont S

Journal:Biophys J

PubMed ID:8527643

'The spatial distribution of depolarized patches of membrane during the excitation of single neurons in culture has been recorded with a high spatial resolution (1 micron2/pixel) imaging system based on a liquid-nitrogen-cooled astronomical camera mounted on an inverted microscope. Images were captured from rat nodose neurons stained with the voltage-sensitive ... More

Voltage-sensitive dye recording of action potentials and synaptic potentials from sympathetic microcultures.

Authors:Chien CB, Pine J

Journal:Biophys J

PubMed ID:1681956

'Given the appropriate multicell electrophysiological techniques, small networks of cultured neurons (microcultures) are well suited to long-term studies of synaptic plasticity. To this end, we have developed an apparatus for optical recording from cultured vertebrate neurons using voltage-sensitive fluorescent dyes (Chien, C.-B., and J. Pine. 1991. J. Neurosci. Methods. 38:93-105). ... More

Charge translocation by the Na,K-pump: I. Kinetics of local field changes studied by time-resolved fluorescence measurements.

Authors:Bühler R, Stürmer W, Apell HJ, Läuger P

Journal:J Membr Biol

PubMed ID:1652643

'Membrane fragments containing a high density of Na,K-ATPase can be noncovalently labeled with amphiphilic styryl dyes (e.g., RH 421). Phosphorylation of the Na,K-ATPase by ATP in the presence of Na+ and in the absence of K+ leads to a large increase of the fluorescence of RH 421 (up to 100%). ... More

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