ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
Zitierungen und Referenzen (3)
Invitrogen™

ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Achieve unparalleled precision and sensitivity in your fluorescence-based applications from FISH to real-time PCR. With a streamlined, non-enzymatic labeling process and a broad selection of vibrant dyes, ULYSIS kits ensure your nucleic acids are ready for any challenge.
Have Questions?
Ansicht ändernbuttonViewtableView
KatalognummerMarker oder FarbstoffAnregung/Emission
U21654Alexa Fluor™ 594588 bis 615 nm
U21650Alexa Fluor™ 488492/520 nm
U21652Alexa Fluor™ 546555/570 nm
U21660Alexa Fluor™ 647650 – 670 nm
Katalognummer U21654
Preis (EUR)
876,00
1 kit
Zum Warenkorb hinzufügen
Marker oder Farbstoff:
Alexa Fluor™ 594
Anregung/Emission:
588 bis 615 nm
Preis (EUR)
876,00
1 kit
Zum Warenkorb hinzufügen

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

Features include:

  • Labeling reaction complete in as little as 15 minutes
  • Available in several Alexa Fluor dye colors
  • Non-enzymatic labeling—avoids the potential biases and limitations associated with enzymatic labeling methods
  • Flexibility—compatible with a wide range of experimental conditions and protocols
  • High sensitivity—provides strong fluorescence signals, enabling detection of low-abundance targets

Reliable labeling with the Universal Linkage System

This series of ULYSIS kits was developed to enable rapid and simple coupling of Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), makes use of a platinum dye complex that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is fast and easy

The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished with a simple spin-column procedure. DNA longer than ∼1,000 base pairs require a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FarbeRed
Anregung/Emission588 bis 615 nm
Zur Verwendung mit (Anwendung)Dot blot, Northern blot, Southern blot, RNA in situ hybridization, DNA in situ hybridization, Multicolor fluorescence in situ hybridization (mFISH), Comparative genome hybridization (CGH), Microarray analysis
Mit Marker oder FarbstoffJa
MarkierungsmethodeDirekte Markierung
ProduktlinieAlexa Fluor, Ulysis
ProdukttypNukleinsäure-Markierungskit
Menge1 Kit
VersandbedingungRaumtemperatur
NachweisverfahrenFluoreszenz
EndprodukttypSonden (markierte RNA), Sonden (markierte DNA), Oligos (markiert)
FormatKit
Labeling TargetDNA (allgemein), Oligos, RNA (allgemein)
Marker oder FarbstoffAlexa Fluor™ 594
ProbentypRNA
Unit Size1 kit
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

View all ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit FAQs

Zitierungen und Referenzen (3)

Zitierungen und Referenzen
Abstract
Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.
Authors:Bates D, Kleckner N
Journal:Cell
PubMed ID:15960977
'Chromosome and replisome dynamics were examined in synchronized E. coli cells undergoing a eukaryotic-like cell cycle. Sister chromosomes remain tightly colocalized for much of S phase and then separate, in a single coordinate transition. Origin and terminus regions behave differently, as functionally independent domains. During separation, sister loci move far ... More
Three-color FISH analysis of TMPRSS2/ERG fusions in prostate cancer indicates that genomic microdeletion of chromosome 21 is associated with rearrangement.
Authors:Yoshimoto M, Joshua AM, Chilton-Macneill S, Bayani J, Selvarajah S, Evans AJ, Zielenska M, Squire JA
Journal:Neoplasia
PubMed ID:16820092
The recent description of novel recurrent gene fusions in approximately 80% of prostate cancer (PCa) cases has generated increased interest in the search for new translocations in other epithelial cancers and emphasizes the importance of understanding the origins and biologic implications of these genomic rearrangements. Analysis of 15 PCa cases ... More
Evaluation of cadmium-induced transcriptome alterations by three color cDNA labeling microarray analysis on a T-cell line.
Authors:Tsangaris GT, Botsonis A, Politis I, Tzortzatou-Stathopoulou F
Journal:Toxicology
PubMed ID:12160620
Beside heavy metals, cadmium (Cd(2+)) is a ubiquitous toxic metal with a well established apoptotic and genotoxic effect, chronic exposure of which has been involved in a variety of pathological conditions. In the present study, we investigated by 1455 genes cDNA microarrays the toxic and apoptotic effect of Cd(2+), on ... More