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      Invitrogen™

      Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

      Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

      Catalog number:  V35123

       Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry | Pharma & Biopharma | Target-Based ADME⁄Tox Assays

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      Description

      This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.

      View a selection guide for all apoptosis assays for flow cytometry.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Flow Cytometer Laser Lines: 405, 488
      Detection Method: Fluorescent
      Excitation⁄Emission (nm): 7-AAD: 546⁄647, PO-PRO™-1: 434⁄456
      For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
      Format: Slide(s), Tube(s)
      Label or Dye: 7-AAD (7-aminoactinomycin D), PO-PRO™-1
      Number of Reactions: 200
      Product Line: Vybrant®
      Product Size: 1 kit
      Shipping Condition: Room Temperature

      Contents & storage

      Contains 1 vial of PO-PRO™-1 dye (500 µL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 µL).

      Store in refrigerator (2–8°C) and protect from light.

      Documents

      Manuals & protocols