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Invitrogen™

Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

Catalog number: V35123
Related applications: Pharma & Biopharma
Cell Viability, Proliferation & Function
Cellular Imaging
Flow Cytometry
Technical SupportCustomer Service
Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry
Invitrogen™

Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

Catalog number: V35123
Related applications: Pharma & Biopharma
Cell Viability, Proliferation & Function
Cellular Imaging
Flow Cytometry
Technical SupportCustomer Service
Catalog Number
V35123
Unit Size
1 kit
Price (USD)
377.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V351231 kit
377.00
Your Price:
***
Product Overview
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This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

Format
Tube, Slide
Product Type
Dead Cell Apoptosis Kit
Flow Cytometer Laser Lines
405, 488
Excitation/Emission
PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647
No. of Reactions
200
Shipping Condition
Room Temperature
Product Line
Vybrant™
For Use With (Application)
Flow Cytometry
Conjugate
7-AAD (7-aminoactinomycin D), PO-PRO™-1
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer

Contents & Storage

Contains 1 vial of PO-PRO™-1 dye (500 µL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 µL).

Store in refrigerator (2–8°C) and protect from light.

Figures

Flow cytometric analysis of Jurkat cells with PO-PRO™-1 dye, and 7-aminoactinomycin D (7-AAD).
Flow cytometric analysis of Jurkat cells with PO-PRO™-1 dye, and 7-aminoactinomycin D (7-AAD).