Invitrogen™

Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

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Description

This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647
Flow Cytometer Laser Lines: 405, 488
Label Type: Other Label(s) or Dye(s)
Label or Dye: 7-AAD (7-aminoactinomycin D), PO-PRO™-1
No. of Reactions: 200
Product Line: Vybrant®
Shipping Condition: Room Temperature
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Format: Tube(s), Slide(s)

Contents & storage

Contains 1 vial of PO-PRO™-1 dye (500 µL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 µL).

Store in refrigerator (2–8°C) and protect from light.