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Invitrogen™
Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry
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Description
This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Flow Cytometer Laser Lines: | 405, 488 |
|---|---|
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 7-AAD: 546⁄647, PO-PRO™-1: 434⁄456 |
| For Use With (Equipment): | Flow Cytometer, Fluorescence Microscope |
| Format: | Slide(s), Tube(s) |
| Label or Dye: | 7-AAD (7-aminoactinomycin D), PO-PRO™-1 |
| Number of Reactions: | 200 |
| Product Line: | Vybrant® |
| Product Size: | 1 kit |
| Shipping Condition: | Room Temperature |
Contents & storage
Contains 1 vial of PO-PRO™-1 dye (500 µL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 µL).
Store in refrigerator (2–8°C) and protect from light.
Store in refrigerator (2–8°C) and protect from light.
