Microscopical examination of the localisation patterns of two novel rhodamine derivatives in normal and neoplastic colonic mucosa.
AuthorsAtlamazoglou V, Yova D, Kavantzas N, Loukas S
JournalLasers Med Sci
PubMed ID11702630
'Tissue characterisation by fluorescence imaging, using exogenous fluorophores, is a promising method for cancer detection. Histochemical alterations in the composition of mucins, when neoplastic transformations occur, could be exploited to derive more selective fluoroprobes indicative of early malignant transformation. The aim of this work was to develop and examine tumour ... More
The early activation marker CD69 regulates the expression of chemokines and CD4 T cell accumulation in intestine.
'Migration of naïve and activated lymphocytes is regulated by the expression of various molecules such as chemokine receptors and ligands. CD69, the early activation marker of C-type lectin domain family, is also shown to regulate the lymphocyte migration by affecting their egress from the thymus and secondary lymphoid organs. Here, ... More
Factor VIII A3 domain substitution N1922S results in hemophilia A due to domain-specific misfolding and hyposecretion of functional protein.
AuthorsSummers RJ, Meeks SL, Healey JF, Brown HC, Parker ET, Kempton CL, Doering CB, Lollar P,
JournalBlood
PubMed ID21217077
A point mutation leading to amino acid substitution N1922S in the A3 domain of factor VIII (fVIII) results in moderate to severe hemophilia A. A heterologous expression system comparing N1922S-fVIII and wild-type fVIII (wt-fVIII) demonstrated similar specific coagulant activities but poor secretion of N1922S-fVIII. Immunocytochemical analysis revealed that intracellular levels ... More
The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP.
AuthorsCha B, Kenworthy A, Murtazina R, Donowitz M
JournalJ Cell Sci
PubMed ID15226406
The epithelial brush border (BB) Na+/H+ exchanger, NHE3, plays a major role in transcellular Na+ absorption in the renal proximal tubule. NHE3 activity is rapidly regulated by neurohumoral substances and growth factors via changes in its amount on the BB by a process partially involving vesicle trafficking. The PDZ domain-containing ... More
Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses.
Authors
JournalCell Host Microbe
PubMed ID23498957
An association between peptidoglycan synthesis and organization of the Streptococcus pyogenes ExPortal.
Authors
JournalMBio
PubMed ID24065630
Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise.
AuthorsAbou Sawan S, van Vliet S, West DWD, Beals JW, Paluska SA, Burd NA, Moore DR
JournalAm J Physiol Cell Physiol
PubMed ID30133322
'We have recently demonstrated that whole egg ingestion induces a greater muscle protein synthetic (MPS) response when compared with isonitrogenous egg white ingestion after resistance exercise in young men. Our aim was to determine whether whole egg or egg white ingestion differentially influenced colocalization of key regulators of mechanistic target ... More
Nutritional Strategies to Offset Disuse-Induced Skeletal Muscle Atrophy and Anabolic Resistance in Older Adults: From Whole-Foods to Isolated Ingredients.
AuthorsMarshall RN, Smeuninx B, Morgan PT, Breen L
JournalNutrients
PubMed ID32466126
'Preserving skeletal muscle mass and functional capacity is essential for healthy ageing. Transient periods of disuse and/or inactivity in combination with sub-optimal dietary intake have been shown to accelerate the age-related loss of muscle mass and strength, predisposing to disability and metabolic disease. Mechanisms underlying disuse and/or inactivity-related muscle deterioration ... More
Characterisation of L-Type Amino Acid Transporter 1 (LAT1) Expression in Human Skeletal Muscle by Immunofluorescent Microscopy.
AuthorsHodson N, Brown T, Joanisse S, Aguirre N, West DWD, Moore DR, Baar K, Breen L, Philp A
JournalNutrients
PubMed ID29278358
'The branch chain amino acid leucine is a potent stimulator of protein synthesis in skeletal muscle. Leucine rapidly enters the cell via the L-Type Amino Acid Transporter 1 (LAT1); however, little is known regarding the localisation and distribution of this transporter in human skeletal muscle. Therefore, we applied immunofluorescence staining ... More
Neuronal FcεRIα directly mediates ocular itch via IgE-immune complex in a mouse model of allergic conjunctivitis.
Authors
JournalJ Neuroinflammation
PubMed ID35197064
Live single-cell laser tag.
Authors
JournalNat Commun
PubMed ID27198043
High-throughput fitness screening and transcriptomics identify a role for a type IV secretion system in the pathogenesis of Crohn's disease-associated Escherichia coli.
Authors
JournalNat Commun
PubMed ID33795670
Host-Specific Adaptive Diversification of Crohn's Disease-Associated Adherent-Invasive Escherichia coli.
Authors
JournalCell Host Microbe
PubMed ID30683582
Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platform.
Authors
JournalNat Commun
PubMed ID33257686
CX3CR1⁺ cells facilitate the activation of CD4 T cells in the colonic lamina propria during antigen-driven colitis.
Authors
JournalMucosal Immunol
PubMed ID24129164
Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID35588457
Z-form extracellular DNA is a structural component of the bacterial biofilm matrix.
Authors
JournalCell
PubMed ID34735796
Fifteen days of 3,200 m simulated hypoxia marginally regulates markers for protein synthesis and degradation in human skeletal muscle.
AuthorsD'Hulst G, Ferri A, Naslain D, Bertrand L, Horman S, Francaux M, Bishop DJ, Deldicque L
JournalHypoxia (Auckl)
PubMed ID27800505
Chronic hypoxia leads to muscle atrophy. The molecular mechanisms responsible for this phenomenon are not well defined in vivo. We sought to determine how chronic hypoxia regulates molecular markers of protein synthesis and degradation in human skeletal muscle and whether these regulations were related to the regulation of the hypoxia-inducible ... More