EGS (etilenglicol bis(succinimidil succinato))

Citas y referencias (5)

Thermo Scientific™

EGS (etilenglicol bis(succinimidil succinato))

El EGS Thermo Scientific Pierce es un entrecruzador que contiene extremos de éster NHS reactivos con aminas alrededor de unMás información

Número de catálogo	Cantidad
21565	1 g

Número de catálogo 21565

Precio (USD)

405,00

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Cantidad:

Pedido a granel o personalizado

Precio (USD)

405,00

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El EGS Thermo Scientific Pierce es un entrecruzador que contiene extremos de éster NHS reactivos con aminas alrededor de un brazo espaciador de 12 átomos, que se puede escindir mediante un tratamiento con hidroxilamina a pH 8,5.

Características del entrecruzador EGS:

• Grupos reactivos: Éster NHS (ambos extremos)
• Reactivo a: grupos amino (aminas primarias)
• Insoluble en agua (disolver primero en DMF o DMSO); compare con Sulfo-EGS
• Membrana permeable, lo que permite el entrecruzamiento intracelular
• Los entrecruzados formados son reversibles a pH 8,5 usando hidroxilamina durante un periodo de 3 a 6 horas a 37 °C
• La lactosa deshidrogenasa retuvo el 60 % de su actividad después del entrecruzamiento reversible con EGS

Referencias de producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Productos relacionados
EGS (etilenglicol bis(succinimidil succinato))

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónEGS

FormularioPolvo

Método de etiquetadoEtiqueta química

Peso molecular456.36

PegiladoNo

Línea de productosPierce

Cantidad1 g

Fracción reactivaÉster NHS

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador16,1 Å

Soluble en aguaNo

Reactividad químicaAmina-amina

CleavablePor hidroxilamina

Tipo de enlace cruzadoHomobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorMedio (de 10 a 30 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (5)

Citations & References

Abstract

Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.

Authors:Keinan N, Tyomkin D, Shoshan-Barmatz V

Journal:Mol Cell Biol

PubMed ID:20937774

'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More

Structure-based analysis of VDAC1 protein: defining oligomer contact sites.

Authors:Geula S, Naveed H, Liang J, Shoshan-Barmatz V

Journal:J Biol Chem

PubMed ID:22117062

'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More

Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes.

Authors:Mokry M, Hatzis P, Schuijers J, Lansu N, Ruzius FP, Clevers H, Cuppen E

Journal:Nucleic Acids Res

PubMed ID:21914722

'Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RNA polymerase II (Pol II), ... More

LXRa regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8.

Authors:Pourcet B, Feig JE, Vengrenyuk Y, Hobbs AJ, Kepka-Lenhart D, Garabedian MJ, Morris SM, Fisher EA, Pineda-Torra I

Journal:Circ Res

PubMed ID:21757649

Activation of liver X receptors (LXRs) inhibits the progression of atherosclerosis and promotes regression of existing lesions. In addition, LXRa levels are high in regressive plaques. Macrophage arginase 1 (Arg1) expression is inversely correlated with atherosclerosis progression and is markedly decreased in foam cells within the lesion. ... More

Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.

Authors:Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D

Journal:J Biol Chem

PubMed ID:21712378

Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More