Genexus™ Library Strips 1 and 2-HD

The Ion Torrent Genexus Library Strips 1 and 2-HD kit for Ion AmpliSeq HD-based panels provides eight each of theMás información

Número de catálogo	Cantidad
A40255	32 Reactions

Número de catálogo A40255

Precio (USD)

Cantidad:

32 Reactions

The Ion Torrent Genexus Library Strips 1 and 2-HD kit for Ion AmpliSeq HD-based panels provides eight each of the strips required to perform automated library preparation using the Ion Torrent Genexus Integrated Sequencer. This kit, combined with Ion Torrent Genexus Barcodes 1-32 HD, supports flexible, fully automated preparation and normalization of up to 32 single-pool Ion AmpliSeq HD libraries.

As part of the Genexus Integrated Sequencer workflow, the Genexus Library Strips 1 and 2-HD kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq HD libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Secuenciación

Para utilizar con (equipo)Genexus™ Integrated Sequencer

BibliotecasTargeted Sequencing Library

N.º de reacciones32

Línea de productosGenexus

Tipo de productoLibrary Strips 1 and 2-HD

Cantidad32 Reactions

Tipo de muestraDNA, RNA

Condiciones de envíoAprobado para su envío en hielo húmedo o seco

Sequencing TypeGenome, DNA, and RNA Sequencing

Unit SizeEach

Contenido y almacenamiento

• Genexus Strip 2-HD, store at -10 to -30°C
• Genexus Strip 1, store at 2–8°C

Preguntas frecuentes

How many samples of Oncomine Precision Assay GX can I run on each lane of the GX5 Chip?

The capacity of the GX5 Chip varies depending on the sample type:
- For plasma cfTNA samples, the workflow is configured to accommodate 1 sample per GX5 Chip lane (or 4 samples on a single GX5 Chip).
- For FFPE DNA+RNA samples, the workflow is configured to accommodate 4 samples per GX5 Chip lane.
- For FFPE DNA-only or RNA-only samples, the workflow is configured to accommodate 8 samples per GX5 Chip lane.

How many sequencing reactions can I perform with Oncomine Precision Assay GX (Cat. No. A44350)?

The maximum number of samples that can be analyzed per kit depends upon the type of analysis that you want to perform. Each kit provides the targeted pancancer panel and library reagents sufficient to perform up to 32 sequencing reactions on the Genexus Integrated Sequencer.

- Simultaneous DNA- and RNA-based variant analysis for up to 16 FFPE tissue samples (one DNA reaction and one RNA reaction per sample)
- DNA-only or RNA-only variant analysis for up to 32 FFPE tissue samples
- Cell-free TNA (cfTNA) variant analysis (combined DNA and RNA) for up to 32 plasma samples

How much of each sample do I need for the Oncomine Precision Assay GX?

For solid tumor samples, the optimal input amount is 10 ng with validated input in the range of 5-15 ng. For liquid biopsy samples, the optimal input amount is 20 ng with validated input in the range of 5-40 ng.

For optimal results, load 20 µL of input with a concentration of ≥0.67 ng/µL for FFPE DNA and FFPE RNA samples and 20 µL of input with a concentration of ≥1.33 ng/µL for cfTNA samples. If the concentration of the sample is lower than optimal, use as much input as possible for each reaction.

What controls should I use with Oncomine Precision Assay GX?

Here are the various controls we recommend depending upon the starting material:

FFPE DNA samples
- Structural Multiplex Reference Standard (FFPE), Cat. No. HD789 (Horizon™)
- EGFR Gene-Specific Multiplex Reference Standard (FFPE) 5% AF, Cat. No. HD300 (Horizon™)
- KRAS Gene-Specific Multiplex Reference Standard (FFPE), Cat. No. HD301 (Horizon™)
- Seraseq® Lung & Brain CNV Mix, + 3 copies, Cat. No. 0710-0414 (SeraCare®)
- Seraseq® Breast CNV Mix, + 6 copies, Cat. No. 0710-0412 (SeraCare®)

FFPE RNA samples
- Seraseq® FFPE Tumor Fusion RNA v4 Reference Material, Cat. No. 0710-0496 (SeraCare®)
- Seraseq® FFPE NTRK Fusion RNA Reference Material, Cat. No. 0710-1031 (SeraCare®)

cfTNA samples
- Multiplex I cfDNA Reference Standard Set, Cat. No. HD780 (Horizon™)

Which sample types can I use with Oncomine Precision Assay GX?

Oncomine Precision Assay GX is compatible with solid tumor samples (FFPE DNA or FFPE RNA) as well as liquid biopsy samples (cfTNA).

View all Genexus™ Library Strips 1 and 2-HD FAQs