ELISA Assay Buffer (5X)
ELISA Assay Buffer (5X)
Citas y referencias (1)
Invitrogen™

ELISA Assay Buffer (5X)

ELISA Assay Buffer (5X) is intended for use as: blocking reagent for ELISA plates, diluent for ELISA standards and samples,Más información
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Número de catálogoCantidad
DS98200200 mL
Número de catálogo DS98200
Precio (CLP)
241.691
Each
Añadir al carro de la compra
Cantidad:
200 mL
Precio (CLP)
241.691
Each
Añadir al carro de la compra
ELISA Assay Buffer (5X) is intended for use as: blocking reagent for ELISA plates, diluent for ELISA standards and samples, diluent for the detection antibody, and diluent for the HRP conjugate. This buffer is recommended when using Invitrogen Antibody Pairs or when developing a sandwich ELISA.

For a complete kit of ELISA buffers, see ELISA Buffer Kit.
View all available buffers and reagents for ELISA applications.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Condiciones de envíoWet Ice
AplicacionesELISA
FormularioConcentrated
Cantidad200 mL
Unit SizeEach
Contenido y almacenamiento
Buffer. Store at 2-8°C.

Preguntas frecuentes

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Citations & References (1)

Citations & References
Abstract
Neutrophil CD11b, L-selectin and Fc IgA receptors in patients with dermatitis herpetiformis.
Authors:Smith AD, Streilein RD, Hall RP,
Journal:Br J Dermatol
PubMed ID:12452859
'The skin lesions found in patients with dermatitis herpetiformis (DH) are characterized by the presence of neutrophils at the dermal papillary tips in areas where the diagnostic cutaneous IgA deposits are found. Although the presence of the skin lesions of DH is known to be associated with gluten-sensitive enteropathy, the ... More